The best method for isolation and purification of - dihydroagarofuran sesquiterpene in celastrus angulatus is alternate normal - phase and reserve - phase high performance liquid chromatography 结果表明,对苦皮藤中富含二氢沉香呋喃倍半萜类化合物部分进行分离的过程中,采用正相、反相高压制备柱层析交替进行的方法最为有效。