English translation for "反义rna"
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- interfering complementaryrna
mrna interfering complemen-tary rna
Related Translations:
- Example Sentences:
| 1. | Transfection of 3t3 - l1 preadipocytes with a c - crkii antisense rna expression vector markedly reduced c - crkii levels and prevented differentiation into adipocytes
将c - crkii的反义rna转化细胞能够显著的降低细胞内c - crkii的表达水平并抑制脂肪细胞的分化。 | | 2. | Research on e . coli protease system is the basis of engineering strain improvement in order to reduce their negative effect , much has been done , among which antisense rna technology is worth further trying
为减少蛋白酶对外源蛋白的降解，从不同的角度做了大量工作，其中利用反义rna技术控制蛋白酶活性是一条值得探索的思路。 | | 3. | On the other hand , maize dwarf mosaic ( mdm ) is a kind of main disease that cause huge drop in production and there is no effective pesticide now . antisense rna of maize dwarf mosaic virus coat protein ( mdmv - cp ) gene can well depress the assemble of coat protein , avoiding mdmv disease effectively
玉米矮花叶病是造成玉米减产的一种重要病毒病害，目前尚无有效的化学药物加以防治，而利用反义rna技术培育抗玉米矮花叶病的转基因玉米是一条防治玉米矮花叶病害的经济、有效的途径。 | | 4. | An expression vector with fragment 9 in antisense orientation was constructed to block the expression of the relevant gene ( fragment 9 related gene , fnr gene ) in vero cells . interestingly , we found that the nontargeted mutation frequency induced by mnng was increased significantly , implicating that the product of the blocked gene may be involved in the inhibition of nontargeted mutation
利用反义核酸技术构建含反向插入9号片段的真核细胞表达重组体并转染细胞，以获得反义rna阻断vero细胞中相应基因的表达，发现mn ’ ng诱发的非定标性突变频率显著增高，提示被阻断的相关基因的表达产物可能参与抑制非定标性突变的发生。 | | 5. | The ul54 mrna sequence encoding hcmv dna polymerase is very important for virus duplication . construction of migss may be a tool that allows us selectively to cleave hcmv ul54 mrna with high specificity and effectiveness in vitro . pgemsz , which contains a t7 promoter region , was chosen as the vector for gene ul54
本课题以人巨细胞病毒( hcmv ) dna聚合酶基因为靶序列，设计构建人工核酶m1gs ，研究对该基因mrna片段的切割作用，为进一步研究以rnasep为基础的反义rna技术抑制病毒基因表达奠定理论基础。 | | 6. | The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action , thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study , we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons
本研究设计以c tamrna为模板的反义cdna片段，从c ta基因5 ’端第94位到500位核苷酸段设计引物，目的片段407bp ，覆盖第116和188位两个aug密码子，也包含了第一外显子和第二外显子间的剪接位点：用常规分子生物学方法构建了反义片段的腺病毒表达载体( padeasy - 1系统) ；腺病毒载体经hek293细胞包装产生含反义片段的重组腺病毒，用氯化铯密度梯度离心法获得纯化的高滴度腺病毒；进行体外基因转移，分别用反义片段真核表达载体转染p388d1细胞和用重组腺病毒感染hela细胞，观察导入的c ta基因反义rna抑制细胞内组成型或诱导型c ta基因表达的作用，从而达到调控mhc -类分子表达的目的。 |
- Similar Words：
- "反意字" English translation, "反义" English translation, "反义2" English translation, "反义dna" English translation, "反义mrna" English translation, "反义rna及核酶介导的抗性" English translation, "反义词" English translation, "反义词词典" English translation, "反义词为" English translation, "反义词游戏" English translation
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