| 1. | Protocol of dot blot and in situ hybridization for white spot virus
对虾白斑病毒斑点杂交和原位杂交检测操作规程 |
| 2. | By northern dot blotting , it also showed that the foreign gene could express in the transgenic plants
Northern斑点杂交表明，外源基因在转基因植株中正常表达。 |
| 3. | To device a primer pairs and amplify the full length pstvd by rt - pcr , the positive rna extraction from tuber sample was used as template . the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector
Cdna核酸斑点杂交反应( nash )检测pstvd方法准确、灵敏度高，一次检测样品数量多，且对于异地样品检测非常方便，是以往其它检测方法的有效补充。 |
| 4. | Pcr , pcr - southern blot analysis , southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome . the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw , which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce
( 3 )细胞核载体pb - adw 、 pbg - adw均采用农杆菌介导法将adw导入莴苣，细胞核转化获得了生长良好的抗性莴苣植株，经pcr 、 pcr - southern 、 southern斑点杂交分析证实， adw基因已整合到莴苣基因组中。 |
| 5. | Lastly by using the technique of dot blot hybridization , the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer . the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe . by comparing the diagnostic ways of nucleotide probe and fc , the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最后，用地高辛随机引物法标记成momp基因核酸探针，斑点杂交检测衣原体基因组dna ，灵敏度可达10pg ，且不能检出其它病原体的核酸。将核酸探针法与补体结合反应法对衣原体感染的诊断进行比较，初步证明该探针具有较高的敏感性与较强的特异性。 |
| 6. | Expression analysis the expression analysis of gharf in different tissues of cotton was carried out by rna dot blotting with ghakf probe labeled with dig . the result showed that the expression of arf gene was mainly in anthers of sterile and fertile , pollen of fertile , corolla rather than in leaves , radicel and ovule
棉花ari ； ’基因的表达分析用地高辛将棉花arf基因标记为探针，与棉花洞a的不育株花药、司有株花药、可育花粉粒、叶片、花冠、胚根和胚珠的总rna进行斑点杂交。 |
| 7. | One was cloning , sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ) , the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot
为了鉴别诊断tgev与prcv及对tgev进行流行病学调查，本研究采用原核表达系统( gst融合表达系统)表达tgev纤突蛋白( s蛋白)中含有b和c抗原位点的多肽，并且制备了非放射性地高辛标记的核酸探针，通过斑点杂交( dot - blot )检测tgev核酸rna 。 |
| 8. | It indicated that 76 . 5 percent of positive frequency of all the plants was achieved by using southern dot hybridization , 87 . 5 percent of the plants with gus positive activity was gotten by pcr southern blotting and 42 percent of the plants with gus positive activity was gotten by southern blotting
转基因卡那霉素抗性植株中， gus表达阳性率为43 ，随机抽样的方法进行杀虫肽和npt基因的pcr检测阳性率均为100 。经过分子生物学检测表明， southern斑点杂交76 . 5植株表现阳性。 |
| 9. | Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china . cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively . total rna extracted from field samples were used for rna dot - hybridization
用侵染马蹄莲的dsmv3末端序列和黄瓜花叶病毒( cmv )的亚基因组启动子区互补dna序列为标记探针，对自然感病的天南星科植物进行rna斑点杂交，并结合双链rna分析、病毒提纯和形态学观察，对杭州等地16属天南星科植物的81个样品进行了病毒鉴定。 |
