English translation for "cloning dna"
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- 克隆dna
Related Translations:
cloning: 基因转殖 克隆克隆繁殖克隆技术克卢克隆化无性繁殖系化无性系化
- Example Sentences:
| 1. | A bt - e . coli shuttle vector pht315 was deleted its replication region of bt , then constructed a novel vector named pht315 - 1 which composed a multiple cloning site , erythromycin and ampicillin - resistance marker and could only replicated in e . coli . used pht315 - 1 , a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324 . sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501 , 333 , 183aas . orfl had 98 % identities with replicating related protein ori43 of bt strain hd263 . the others were no homology to any published bt replicating related protein . after continuous cultured for 70h at 30 c without antibiotic selecting press . the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 % . and growth curve also showed that the novel replicon was stable and could replicate normally
进一步序列分析表明该复制区至少有3个较大的orf ，分别编码501 ， 333 ， 183个氨基酸。其中orf1蛋白序列与hd263复制蛋白ori43的同源性为98 ，而另外两个orf和genbank己公布的bt复制相关蛋白无同源性。 30连续培养72h ，复制区质粒在bt无晶体突变株hd73cry ~ -中稳定性达98以上， 30h生长曲线也表明该复制区能够在bt中稳定复制和遗传，对受体菌株无明显不良影响。 | | 2. | After enzyme restriction and sequencing analysis , the nucleotide data had been further analyzed by antheprot 5 . 0 and clutalw softwares . the analysis results showed that the cloned dna fragment had a longest open reading frame ( orf ) of 1035nt , it predicted to be encoded a 344 - aa protein with the molecular weight of 36kda
应用antheprot5 . 0 、 clustalw等分子生物学软件分析，显示主要外膜蛋白前24个氨基酸是较强的疏水性区域，可组成信号肽，其与omp基因的同源率达96 ，氨基酸的同源率高达98 。 | | 3. | To prove that the cloned dna fragment can express tryptopanase , a new plasmid pet28c - tnaa , in which the cloned dna fragment was located downstream of t7 promoter on pet28c was constructed and transformed into host bl21 ( de3 ) , a bl21 lysogen of bacteriophage de3 in which the only promoter known to direct transcription of the t7 rna polymerase gene is the lacuvs promoter , which is inducible by iptg
用iptg诱导表达t7rna聚合酶，以表达质粒上的目的基因。在葡萄糖存在的条件下，用常规方法发酵和诱导( 37 1mmiptg ) ，发现表达的蛋白质条带的分子量与理论上计算的分子量一致。但是发酵液中检测不到吲哚，表明虽然表达了目标蛋白，但表达的蛋白质没有酶活性。 |
- Similar Words：
- "cloning (programming)" English translation, "cloning and mutation" English translation, "cloning animals and plants" English translation, "cloning by expression" English translation, "cloning cyndi" English translation, "cloning efficiency" English translation, "cloning equipment" English translation, "cloning fidelity" English translation, "cloning host" English translation, "cloning human embryos" English translation
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