| 1. | Gel filtration chromatography , gfc
凝胶过滤色谱法 |
| 2. | Gel filtration chromatography
凝胶过滤色谱法 |
| 3. | Sds - page and sephcryl s - 200 gel filtration detected the molecular weight of dnaase
利用sds page及sephacryl 200凝胶过滤测定分于量。 |
| 4. | The purified badh , which became a single band in sds - page , has been obtained by a combination of ammonium sulfate fractionation , ion exchange chromatography and gel filtration chromatography
辽宁碱蓬甜菜碱醛脱氢酶分子量为129kd ，单个亚基的分子量为64 . 5kd ，表明其为同型二聚体。 |
| 5. | By gel filtration on sepharose cl - 6b and cellulose acetate pellicle electrophoresis test , hpa and hpb were respectively a single strait symmetry apex and the result of electrophoresis assume a single spectrum strip
经sepharosecl - 6b柱层析和醋酸纤维素薄膜电泳检验， hpa和hpb均为单一狭窄对称峰且电泳呈单一谱带。 |
| 6. | At last , we got the pure protein with the determination of electrophoresis . the molecular weight of the enzyme is estimated at 30903 through gel filtration and sds - page electrophoresis . the isoelectric point is 8 . 6 ? . 3
经sds凝胶电泳和凝胶过滤层析两种方法测定该酶的分子量分别为33113dalton和28840dalton ；其等电点pi = 8 . 6 0 . 3 。 |
| 7. | It was very slow at 5 but become higher above 25 . the enzyme from abomasums used in cheese production was rough extracts containing chymosin and pepsin . through the gel filtration chromatography and ion exchange chromatogra
羔羊胃蛋白酶最适凝乳温度为45 ’ c ；在45处理30inin ，酶活性开始下降， 60c处理30lliln ，酶活性完全丧失；酶的最适ph为1 |
| 8. | The apparent molecular weight of g6pd , determined by gel filtration , was 220kd ; the subunit molecular weight was 50 . 5kd as determined by sds - page , suggesting that the native enzyme was a tetramer consisting of four identical subunits
凝胶过滤法测定该酶表观分子量约为220kd ， sds - page测定亚基分子量约为50 . 5kd ，可见该酶是由四个相同亚基构成的四聚体。 |
| 9. | The purified lectin showed a single protein band on sds - page , and the subunit molecular weight was 12kd . the molecular weight was 45kd determined by gel filtration on sephacryl s - 100 . the result implied that there are four same subunits per lra
经sds - page检测为单一蛋白带，亚基分子量为12kd ， sephacryls - 100凝胶过滤测得其表观分子量为45kd ，表明lra是由四个相同亚基组成的蛋白。 |
| 10. | The enzyme is homologous dimmer with molecular mass 102600 as determined by gel filtration on hplc and subunit mass 52042 as determined by maldi - tof mass spectrometry . the optimal ph of d - hydantoinase is at near 9 . 5 and the optimal temperature is at 45
通过对其生化性质的研究表明，其单体m _ r为52042 ，天然m _ r为102600 ，最适ph为ph9 . 5 ，最适温度为45 ，不同的二价金属离子对酶活性有不同的影响。 |
