| 1. | Depending on the conserved amino acids of glycine betaine abc transporter , a pair of degenerate primers were designed
在h . trueperi发现有甘氨酸甜菜碱abc转运系统。 |
| 2. | So genes implicated in expressing glycine betaine are considered as the most important and the most promising plant salt - tolerant genes
因此甜菜碱合成酶基因被认为是最重要和最有希望的盐胁迫抗性基因。 |
| 3. | Many diverse species of plants accumulate glycine betaine ( glybetaine ) in response to various stresses , and the biosynthesis and accumulation of glybetaine are thought to contribute to various stress
许多植物在受到各种胁迫时，都会引起甘氨酸甜菜碱的积累，甘氨酸甜菜碱的生物合成与积累是植物对胁迫的一种适应机制。 |
| 4. | Depending on the degenerate primers which were designed according to the conserved amino acids of glycine betaine secondary transporter , a fragment about 1 kb was obtained by pcr . the pcr fragment was purified and labeled with dig as a probe
将其总dna用sau3ai部分酶切后，收集4 15kb大小的酶切片段，克隆到用bamhi酶切的puc18中，构建halobacillussp . d8基因组文库，共获得约9000个重组质粒。 |
| 5. | Choline dehydrogenase ( cdh ) isolated from escherichia coli , can help the expression of glycine betaine efficiently . to get some salt - tolerant plants , the cdh gene , mediated by agrobacterium , was transformed to the haploid of populus simoniixp . nigra
本实验以农杆菌( agrobacteriumtumefaciens )为介导进行遗传转化，将编码cdh的耐盐基因( bet - a )导入小黑杨( populussimonii p . nigra )单倍体植株( haploid )的基因组内，以期获得耐盐植株。 |
| 6. | Then , a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer . combined with the opuaa - up , a 2 . 3 kb fragment was obtained through pcr . blast result showed a fragment which contained the partial opuaa , the whole opuab and partial opuac sequences were obtained
再次，根据甘氨酸甜菜碱atp转运系统底物结合蛋白的氨基酸保守序列设计下游简并引物，与atp结合蛋白的上游简并引物组合，经pcr扩增获得2 . 1kb的条带，测序后通过blast比较，结果显示获得atp结合蛋白基因的部分序列、通透酶的全部编码序列和部分甘氨酸甜菜碱结合蛋白基因的核苷酸序列。 |
| 7. | Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity . a partial fragment of the glycine betaine transporter beth gene was obtained from the genome of h , trueperi with degenerate primers . through southern blot hybridization and inverse pcr , a 5 . 1 kb ecori fragment containing the beth gene was sequenced
将扩增片段用地高辛标记成探针，与用不同限制性内切酶完全酶切的h . trueperi总dna片段作southern杂交，结果显示在ecori酶切片段的5 . 1kb处有阳性信号。 |
| 8. | Consequently , study on the mechanisms of salt - tolerance in plants and breeding of salt - tolerant plants has become a key issue in plant biology nowadays . as an important plant osmotic regulators , glycine betaine in plants , under the osmotic stress , can be accumulated in order to give the plants high salt - tolerance by reducing osmotic pressure and maintaining proteinic structure in cell
甘氨酸甜菜碱( glycinebetaine )是一种重要的调渗物质，在盐胁迫下有些植物能通过体内甘氨酸甜菜碱的积累保护细胞内蛋白质的结构和功能，降低细胞水势，从而来增加自身的耐盐能力。 |
