| 1. | Additional homologous sequences of alligator mississipiensis and caiman crocodilus from genbank were aligned together and compared with chelonia mydas
结果显示：在鳄类动物中，扬子鳄与密西西比鳄的亲缘关系最近。 |
| 2. | Kingi and l . marginatoides . out of the aligned homologous sequence of 550 sites obtained , the parsimony informative sites are 97 and occupy 17 . 6 %
比对得到550个位点的同源序列，其中简约性信息位点97个，约占全部位点的17 . 6 。 |
| 3. | No homologous sequence was found by comparing it with the sequences obtained from the net , so that the cloned gene was supposed to be an unknown new gene , designated as opra
同源比较分析没有发现同源序列，推测这一序列可能是一未知的新基因，命名为apra 。 |
| 4. | Angiogenin ( ang ) is a small monomer which universally exists in tissues and cells . angiogenin belonging to ribonuclease superfamily has 35 % homologous sequences with ribonuclease a ( rnase a )
血管生长因子( angiogenin ， ang )是一个小的单聚蛋白，与rnasea是同系物，在组织和细胞中分布广泛。 |
| 5. | Chomez and colleague also found this gene by homologous sequence splicing in the database of gene bank in 2001 and named as mage - hi , but there are no any biological functions of restin reported
2001年， chomez等通过同源序列筛选genbank数据库，也发现了该基因，并命名为mage - h1 ，但有关该基因的具体生物学功能研究还没有报道。 |
| 6. | Rna silencing is a common phenomenon of rna degradation that is induced by homologous sequences . virus and transposon invasions and various kinds of aberrant rnas can provoke rna silencing
Rna沉默是生物体中普遍存在的一种由同源序列引起的rna降解过程，病毒或转座子入侵、以及体内产生的各种异常结构rna都可能成为诱导rna沉默发生的因素。 |
| 7. | By blasting the homologous sequences in genbank databases , the sequence of grass carp gh cdna from pituitary is 98 % homologous compared with the previously cloned gh cdna of grass carp . the cgh cdna fragment was inserted into pgex - 4t - l to construct the expression plasmid . the recombinant plasmid was digested by bamh i and ecor i to identify whether the cgh cdna fragment was inserted into the plasmid , the pgcgh was transformed into e . coli bl21 competent cells
将得到的序列在genbank和embl数据库中进行了同源比较，结果显示：本研究克隆到的草鱼gh基因与genbank中登记的x60474草鱼gh基因有12个碱基的差异，编码的氨基酸有3个氨基酸残基的差异，同源性为98 ，影响蛋白质高级结构的保守二硫键为2个。 |
| 8. | The percentage of polymorphic sites , degree of genetic polymorphism and genetic distance were compared and the phylogenetic tree was constructed by neighbor - joining method . the partial mitochondrial 16s rrna gene was amplified by polymerase chain reaction ( pcr ) and the pcr products were directly sequenced after purified . these sequences , together with the homologous sequences of another trichiuridae species lepidopus caudatus obtained from genbank were used to analyze nucleotide difference and to establish a upgma phylogenetic tree by means of biological informatics
汝us价ay1830 )各12个个体进行rapd分析，对比多态位点比例、遗传多态度以及遗传距离，并构建neighbor - join吨系统树;通过pcr扩增出线粒体165rrna基因，纯化后直接测序，利用生物信息学方法进行序列分析和核昔酸变异比较，结合ge红bar止中大西洋叉尾带鱼( lepid (护脚caud玫tuseuphrasen1788 )同源序列构建u甲cm叭系统树。 |
| 9. | In this paper , phylogenetic relationship of 13 species involved in 6 genera of cruciferae wer e carried out through both the clones of homologous sequences with the primers designed on the basis of conserved regions of cyp86mf gene in cytochrome p450 gene superfamily and the differential analyses of them . meanwhile , complete sequences of some genes in cytochrome p450 gene superfamily were isolated and identified by smart pcr - race strategy , and expressed in e . colt . the results were as follows : ( 1 ) isolated by pcr from 11 species of cruciferae , eleven homologous gene segments that deduced amino acids were identities of over 80 % at nucleotide sequence level and similarities of over 70 % at amino acid sequence level
本论文以已知的细胞色素p450基因超家族成员cyp86mf基因的保守区设计引物对十字花科重要蔬菜作物的6个属13个物种进行了同源序列的分离克隆，通过核酸序列的差异比较分析，研究了该基因在不同物种中的进化关系；同时，通过保守引物的pcr扩增和race相结合的方法对十字花科植物不同物种的细胞色素p450基因家族成员基因全长进行了分离克隆、鉴定和原核表达的研究，获得如下研究结果： ( 1 )通过pcr从十字花科植物不同物种中扩增到11个可以推导出完整氨基酸序列的同源片段。 |
| 10. | The complete nucleotide sequence of the mitochondrial genome of f . limnocharis was detailedly compared with those of 5 other amphibians . the nucleotide sequences of 22 trna encoded by 6 amphibians mitochondrial genomes were combined and aligned to the homologous sequences of the 11 veterbrate taxa . using teleosts as outgroup , the phylogenetic analyses results show that mp , nj and ml trees all strongly support the monophyly of living amphibians with respect to other living tetrapods and favor a sister group relationship for caecilians and salamanders
我们在测定了泽蛙线粒体全基因组序列的基础上，与已知其它的5种两栖类进行详细的比较分析，同时选择了11种高等脊椎动物的线粒体全基因序列，以硬骨鱼类做外群，用22个trna基因合并数据进行系统发生重建分析，结果表明mp 、 nj和ml树都强力地支持现生两栖类动物为单系群并且蝾螈类和蚓螈类为姐妹群关系(自引导值分别为92 、 99 、 100 ) 。 |
