| 1. | The design method of heavy metal interaction experiments basing on half growth inhibition concentration ( ic50 ) and common safety concentration , a new method of heavy metal interaction experiment design method was proposed
重金属交互作用试验设计的方法运用半抑制浓度和公共最大非抑制浓度两个参数，提出了一种重金属交互作用试验设计的方法。 |
| 2. | The ic50 of the active agent purified by carbon column is 0 . 525 mg / ml , which is better than that of acarbose ( ic5o = 0 . 9mg / ml ) . the zymosis juice of w - 20 can greatly decrease hyperglycemia level of mice by alloxan in glucose tolerance test
该组分对ph稳定，在叫叶范围内，抑制率80 ：对热相对较稳定，在90以下仍有75的抑制率。过柱后的粗组分icso约为0 |
| 3. | 2 . mouse lung vessel endothelial cell was used for examining ha , hb and hpd ' s ic50 concentration under saturated light dose and ic50 light dose in saturated concentration irradiated by copper vapor laser of 510 . 6nm and 578 . 2nm separately
应用体外培养的小鼠肺血管内皮细胞系( 1h11 )测定在饱和光剂量的条件下，实验光敏剂对血管内皮细胞的杀伤作用，得出其半数杀伤浓度。 |
| 4. | In vitro endothelial proliferation inhibiting activity of recombinant angiostatin was examined with mtt method by using human umbilical vein endothelial cells ( huvec ) . in this test we could draw the inhibition curve and calculated the ic50 to confirm its bio - activity further we do cam vascular inhibition test in vivo
4验证重组蛋白angiostatin的生物活性：采用mm法测定重组蛋白对原代培养的人脐静脉内皮细胞（ huvec ）的抑制作用，绘出抑制曲线，并计算出ic 。 |
| 5. | Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry . the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines , and compared with pgp inhibitor quinidine . the pgp expression of mcf - 7 adr was strongly positive , the positive rate was 15 % ; the pgp expression of mcf - 7 was negative , the positive rate was 1 . 8 % . ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115 . 2g ml and 207 . 6g ml respectively . ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129 . 8mol l 42 . 1g ml and 94 . 1mol l 30 . 5g ml respectively . tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7 , but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly . immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively . tea polyphenol is not only an anti - tumor agent , but also a multidrug resistant modulator similar as quinidine . tea polyphenol is advantageous for its little toxicity in tumor treatment
用免疫组化法和流式细胞仪对肿瘤细胞系mcf - 7和mcf - 7 adr的p -糖蛋白表达水平进行定性定量研究。用噻唑蓝比色法mtt研究茶多酚的细胞毒性及其对耐药性的逆转作用，并与pgp抑制剂奎尼定进行了比较。免疫组化法检测p -糖蛋白表达水平， mcf - 7 adr呈强阳性，而mcf - 7呈阴性流式细胞仪定量检测结果mcf - 7 adr细胞系细胞阳性率为15 % ， mcf - 7细胞系细胞阳性率为1 . 8 % 。 |
| 6. | 2 . to know the injuring and photobleaching properties of two screened photosensitizer , i . e . hypocrellin a ( ha ) and hypocrellin b ( hb ) using haematoporphyrin derivative ( hpd ) as their control and to measure their ic50 concentration at the saturated light dose and ic50 light dose in saturated concentration
2以血卟啉衍生物( hpd )为对照，测定上述筛选出的光敏剂?竹红菌素( ha ， hb )在饱和光剂量条件下，光敏剂半数杀伤浓度和在饱和光敏剂剂量条件下半数杀伤光剂量，了解ha 、 hb的杀伤效率和漂白特性。 |
| 7. | Results we construct recombinant angiostatin baculovirus with a high virus titer ( 2 + 108 pfu / ml ) successfully . recombinant angiostatin was effectively expressed in insect cells ( sf9 ) as 53 kd fusing protein and its expression level was about 90 % of insect cellular total soluble proteins . the recombinant angiostatin protein could inhibit endothelial cell proliferation in vitro with ic50 value of 2
实验结果我们成功地构建了滴度高达2x10 ‘ pm llil的angiostatin重组? 2 ?杆状病毒，并在昆虫细胞sffi中高效表达了分子量为53kd的an giostatin重组蛋白，重组angiostatin蛋白不仅在体外显著抑制内皮细胞的生长， k 。 |
