| 1. | An internal segment of the whig gene of s . griseus was amplified from plzl through pcr . the 304bp dna fragment was inserted into the ecori / bamhi site of e . coli - streptomyces shuttle plasmid pkc1139 , generating pkc1139 : : a whig , named plz107 , for gene disruption
Pcr法克隆whig基因内部304bp片段，连接到大肠杆菌-链霉菌穿梭质粒吓0139 ，构建了基因阳坏用重组质粒贼q139 ： ：凸mg ，命名plz107 。 |
