English translation for "klac"

克拉奇

Example Sentences:

	1.	Cloning and expression in e . coli of lactase . specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis . klac gene was amplified by pcr and subsequently cloned 乳糖酶基因的克隆及原核表达以乳酸克鲁维斯酵母( kluyveromyceslactis )菌株k538的基因组dna为模板，设计引物，利用pcr获得乳糖酶基因klac ，经dna测序验证，得到克隆t1549 。
	2.	Klac gene was inserted into the vector ppic9 to construct recombinant plasmid p1c9154983 , and then pic9154983 was transformed by electricity pulse into gs115 , pichia pastoris acceptor . after screening , we obtained recombinant yeast 1 # , 8 # and 24 # , that is , klac gene was integrated into the genome dna of gs115 . by detecting the lactase activity , we knew that the klac gene was expressed in vivo of pichia pastoris , but the lactase was not secreted 三个重组菌株经甲醇诱导培养未检测到分泌的乳糖酶，但胞内均检测到有活性的乳糖酶，分别为0 . 1392u / ml 、 0 . 3702u / ml和0 . 2214u / ml ，说明乳糖酶基因klac在巴斯德毕赤酵母中获得表达。
	3.	So the lactase was suitable to whey at ph7 . 0 , but could n ' t react at high tempreture . the metals , fe2 + , mn2 * , ca2 + , mg2 + and zn2 + , can prominently improve lactase activity , but cu2 + would strongly inhibit the activity . according to the effect of edta , we concluded that the lactase depends on metal ions in a certain extent 乳糖酶基因klac在巴斯德毕赤酵母中表达将klac基因插入到载体ppic9上构建重组表达质粒pic9154983 ，电击转化巴斯德毕赤酵母受体菌gs115 ，经筛选和pcr鉴定， 1 # 、 8 #和24 #为重组菌株， klac基因整合到gs115基因组中。
	4.	Klac gene was ligated to the pet - 30a ( + ) vector for expression . then the recombinant plasmid petlac4 was transformed into e . coli bl21 . the positive transformants were induced at different temperature for three hours by iptg with the final concentration of lmm . sds - page analysis and lactase activity assay showed that klac gene was expressed in e . coli , and that the expression level at 28 was much higher than that at 37 表达产物的sds - page分析和酶活性测定表明， klac基因在大肠杆菌中获得活性表达，其中低温条件28下的表达水平明显高于37 ， 28诱导的细胞经超声波破碎所测酶活力为0 . 475u / ml ， 37仅为0 . 134u / ml 。

Similar Words：

"klabunde" English translation, "klabunn" English translation, "klabunovski" English translation, "klabunovsky" English translation, "klabzuba" English translation, "klacar" English translation, "klacek" English translation, "klacer" English translation, "klachau" English translation, "klachko" English translation