| 1. | Rapid detection of dengue viruses by multiplex pcr assay
技术快速鉴定登革病毒 |
| 2. | Research of genetically modified rapeseed detection with multiplex pcr - gene chip
芯片联用检测方法的研究 |
| 3. | Rapid detection of enterohemorrhagic escherichia coli in food with multiplex pcr assay
快速检测食品中肠出血性大肠杆菌 |
| 4. | The y - a489 - plex multiplex pcr is feasible for using home product of taq dna polymerase
Y一a489一plex体系采用的是国产的taq酶。 |
| 5. | Result a multiplex pcr system of four y - str loci ( y - a489 - plex ) was established . here , y - a489 means the selected loci dys434 , dys438 , dys439 , a10
在100个样本中检出37个单倍型，单倍型变异度为0 . 9628 ，标准误为0 . 00429 。 |
| 6. | Also , these str loci were amplified by multiplex pcr using our primer set , but the universal primers were fluorescent - labeled . the products of the multiplex pcr were genotyped by abi prism 310 genetic analyzer
结果通过采用公共引物和基因座特异性嵌合引物的方法，建立了法医str基因座嵌合引物复合扩增方法。 |
| 7. | The tailed primer design protocol is feasible for y - str loci and can be used to develop more multiplex pcr assays of y - str loci . the results of validation prove that the y - a489 - plex multiplex pcr system can be accepted for the forensic dna typing
证明y一a489一plex ，适合于法医dna分析，有较高的应用价值，为法医y染色体分型提供了新的技术手段。 |
| 8. | In order to make y - str markers more widely accepted , population studies and robust assays are required . we focus on developing new multiplex pcr for y - str loci that can be detected by sliver staining system and fluorescent system
本课题旨在通过研究复合扩增技术，构建四个y - str基因座的复合扩增银染检测体系和复合扩增荧光检测体系，并对其进行法医学可行性研究和群体遗传研究。 |
| 9. | Furthermore , the home product of taq dna polymerase had the same specificity and efficiency compared to the amplitaq gold ( pe , usa ) in this study . conclusion this is the first time that the tailed primer design protocol for multiplex pcr system is used for y - str loci
初步构建了四个y一str基因座的y一a489一plex银染体系和y一a4sg一plex荧光体系:并依据dna分析技术工作组( twgdam )指南进行了应用性研究。 |
| 10. | Result . a method of multiplex pcr based on both chimeric and universal primers was established . a fluorescent quadriplex str system , including d1s1612 , d9s2026 , d20s161 and d6s477 , was developed on the basis of the multiplex pcr
建立了dis1612 、 d952026 、 d205161 、 d65477str基因座荧光标一记嵌合引物复合扩增毛细管电泳激光自动分析体系，同时证明嵌合引物复合扩增方法与毛细管电泳激光自动分析方法相兼容。 |
