| 1. | Restriction fragment length polymorphism . pcr - rflp
限制性片段长度多态性 |
| 2. | Polymerase chain reaction - restriction fragment length polymorphism , pcr - rflp
限制性片段长度多态性 |
| 3. | Polymerase chain reaction and restriction fragment length polymorphism , pcr - rflp
限制性片段长度多态性 |
| 4. | 1983 restriction fragment length polymorphism ( rflp ) technique used in cancer research
1983年,限制片段长度多态( rflp )技术在癌症研究中应用 |
| 5. | Mouse ; restriction fragment length polymorphism ; dna fingerprint ; probe jl - 02 ; es cell
小鼠限制性片段长度多态性dna指纹图jl - 02探针胚胎干细胞 |
| 6. | 1 . because the taxonomic division is rather complex and has been much disputed and revised , in this part , we will review the classification and phylogeny of families , subfamilies and tribes of anseriformes based on morphology , ethology , osteology , mitochondrial and nuclear dna restriction fragment length polymorphism , single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition , classification and phylogenetic relationships of the families , subfamilies and tribes of anseriformes . the controversial questions and deficiency in the systematic studies of anseriformes were pointed out
具体包括以下几个部分： 1 、针对雁形目鸟类异常复杂的分类状况及分类上存在的争议，根据雁形目鸟类的形态学、行为学、骨骼学、角蛋白、线粒体与核dna酶切片段长度多态、单拷贝核dna - dna杂交及线粒体基因dna序列分析等方面的研究，对雁形目鸟类分类中科、亚科和族的划分及其相互间的系统发生关系进行综述，分析系统学研究中存在的不足，提出了雁形目鸟类分类中急需解决的问题。 |
| 7. | All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene . this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )
所有的样本通过聚合酶链式反应?限制性片段长度多态性方法( pcr - rflp )对ahsg基因的第7个外显子内的sac多态性位点进行基因分型，该多态性位点为238号氨基酸密码子中间的碱基c到g的替换，使苏氨酸( thr ， acc )变为丝氨酸( ser ， agc ) 。 |
| 8. | The antigenic and genetic variability of porcine reproductive and respirators syndrome virus ( prrsv ) isolates in china were studied by immunofluoresent monolayer assays ( 1fma ) and restriction fragment length polymorphism ( rflp ) of reverse transcription ( rt ) and polymerases chain reaction ( pcr ) amplified - prrsvorfs fragments among 8 chinese isolates
本研究通过对猪繁殖与呼吸综合征病毒( prrsv )国内分离毒株的gp3 、 gp5和n蛋白的抗原性比较及其orf5和orf7遗传变异性分析，系统研究了国内分离毒株的抗原特性和遗传学差异。 |
| 9. | Analyse the research of distributing of microbe community and the tendency of the change , disscuss the principle and traits of denaturing gradient gel electrophoresisand terminal restriction fragment length polymorphism , to research the law of change that the microbe community have in composting process , we can get effective and rapid information to filtrate the microorganism during composting process , then accelerate the development of compost technology
摘要对堆肥微生物种群分布及其动态变化的研究进行了分析，论述了分子生物技术中的变性梯度凝胶电泳和末端标记限制性片段长度多态性的原理和特点，以及用于研究堆肥微生物的群落结构演变规律，为分析和筛选堆肥中的微生物提供更加有效、快速的信息，促进堆肥技术的发展。 |
| 10. | Hbv genotype was determined by the restriction fragment length polymorphism analysis in patients with chronic hbv infection in 5 cities of fujian province . 2 . the sensitivities and specialties of melting curve assay and pcr microplate hybridization - elisa assay were compared with mpcr - rflp and sequence analysis for the detection of hbv ymdd mutants in 44 serums from patients receiving lamivudine monitherapy with viral breakthrough
应用熔解曲线法和pcr微板核酸杂交- elisa法对44例接受拉米夫定治疗过程中出现病毒学反跳时的血清进行ymdd突变株的检测，并与测序法和mpcr - rflp法比较它们的敏感性、一致性。 |
