| 1. | In this case , two restriction sites ( ecor i , hind iii ) were added to the primers ( p1 , p2 ) separately
扩增产物经双酶切后，重组到表达载体pet - 32a ( + )中。 |
| 2. | Oligomap - oligomap is a tool to help researchers engineer restriction sites into oligonucleotides using silent mutagenesis and reverse translation algorithms
Oligomap是一个帮助研究人员使用哑诱变和反向转换算法来约束寡核苷酸位置点的工具。 |
| 3. | 2 . ecori / bamhi digested pcr products were inserted into the corresponding restriction site in the expression plasmid pbv220 . construction of non - fusion expression plasmid pbv220 - endostatin
用ecori和bamhi酶切endostatincdna的pcr产物，将其插入到质粒载体pbv220中相应的限制性酶切位点，构建非融合质粒表达载体pbv220 - endostatin 。 |
| 4. | A pair of primers containing sph i and hind iii restriction sites were designed , according to the poifn - a gene in ddbj / genbank . then poifn - a gene was cloned from porcine genomic dna by pcr
根据ddbj genbank基因库中已登录的猪干扰素基因序列，设计了含sph和hind酶切位点的一对引物，采取聚合酶链式反应( pcr )法，以猪基因组dna为模板进行了poifn克隆。 |
| 5. | Ba - dfe mature peptide - coding sequence was cloned to the restriction site ncol and ndei of the expression vector pet - 15b , respectively , and highly expressed in e . coli bl21 ( de3 ) by the form of non - fusion and his - badfe fusion protein
Lmmol liptg诱导时，融合表达时产生分子量约为30kd 、带有his标签的ba dfe融合蛋白，非融合表达时则产生分子量为28kd的ba dfe 。 |
| 6. | 6 . the restriction analysis of its amplified product showed that no restriction site was observed for bamh i in all isolates , the other tested enzymes ( alu , hae iii , hinf i , taq i , hha i , msp i ) could distinguish p . cilrinopileatus from the other pleurotus species
6 . [ ts扩增产物的限制性酶切分析结果表明，召til ) ， hl对所有供试菌株均无酶切位点，而另外6利，供试内切酶( alul 、 haeitl 、 llllal 、 11infl 、九式斗， i 、 tcl叮i )都能将金顶侧耳与其它侧耳菌株区分开。 |
| 7. | Total rna was extracted from hepatic cells of mouse . a ecori and bamhi restriction sites were introduced into endostatin gene at specific primer f r , and endostatin was amplified by rt - pcr , this endostatin gene contained bamhi and ecori restriction sites at its 5 " and 3 " ends respectively
从小鼠肝脏细胞中提取总rna 。设计合成一对特异引物，分别带有ecori和bamhi的限制性内切酶的识别位点。用rt - pcr法扩增endostatin的基因片段，在endostatin基因的两侧引入ecori和bamhi酶切位点。 |
| 8. | Sinensis and e . j . hepuensis has been found in the sequences of the portions of 16s rdna and pcr / rflp studies of 110 samples , from six river valleys in eastern mainland of china . these subspecies - specific restriction sites allow rapid discrimination with the endonuclease dra i , and therefore can be used as a diagnostic genetic marker for identification of the two subspecies
通过对中国大陆东部6个水系110个绒螯蟹个体16srdna部分序列的测定和pcr rflp分析，发现在合浦绒螯蟹与中华绒螯蟹之间存在3 4个固定的碱基替代，这种亚摘要种特异性的限制性位点可以通过限制性内切酶dra进行快速检测，成为2个亚种的分子鉴定标记。 |
| 9. | 2 . construction of chimeric mtb8 . 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8 . 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8 . 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments , respectively
3 .重组质粒在真核细胞中的表达: pm 、 pms 、 pmi和pmsl重组质粒用lipofectaminatmzo0o脂质体转染试剂转染cos一7细胞，进行瞬时表达， 48小时后，用rl ’ - pcr检测目的基因在mrna水平的表达;用westemblotting检测hil一12在蛋白质水平的表达。 |
