| 1. | Heredity and expression of foreign genes in pin transgenic flowering chinese cabbage obtained by vacuum infiltration
基因菜薹外源基因的遗传与表达 |
| 2. | Several factors influencing the vacuum infiltration transformation efficiency in brassica campestris l . ssp
对小白菜真空渗入转化法的转化条件进行了初步探索。 |
| 3. | Chinensis were also studied . the developmental status of seedlings , vacuum infiltration time , type of agrobacterium tumefaciens used , all influence the transformation frequency
结果表明植株生长阶段和真空渗入处理时间，所用菌株类型均对转化率有影响。 |
| 4. | It was concluded that using the nopaline type of agrobacterium ( e . g . c58 ) , 5min vacuum infiltration , and using the plants with just several blooming flowers would give the best results
其中以开花初期的植株，利用胭脂碱型农杆菌介导(如c58 ) ，真空渗入5分钟的处理方法转化效率最高。 |
| 5. | 3 . arabidopsis plants were transformed with the constructed plant binary expression vector . eighteen independent antibiotic - resistant arabidopsis transformants for dreb1c were obtained by using a vacuum infiltration method
载体全长为13kb ，有一个烟草花叶病毒35s强启动子和nos终止序列，利用bamhi和sacl做为酶切位点，构建载体。 |
| 6. | Restriction enzyme digestion analysis and pcr analysis showed that these two plant expression vectors constructed were correct . 49caixin , one cultivar of the brassica campestris l . ssp . chinensis , was transformed by vacuum infiltration method
通过真空渗入和花序浸渍法，将上述双抗和单抗基因分别导入白菜不结球类型? ? 49菜心中，获得了7棵抗性株。 |
| 7. | They indicated that ecbp21 may have some relative with the response to stress treatments . further more , we constructed the binary vector containing ecbp21 - gfp chimeric gene and got transgenic plants by using the agrobacterium vacuum infiltration method
4 .初步开展了转基因研究:为了进一步确定ecbp21在植物生长发育过程中的作用，构建了ecbp21植物表达二元载体，通过真空渗透法转化了拟南芥并获得了转基因植株。 |
| 8. | This thesis fabricated successfully a wc particle reinforced iron matrix composite using a vacuum infiltration process for severely erosion and application in the slurry pump . the casting process was optimized . the microstructure and wear resistance were researched and analyzed
本文针对承受严重冲蚀磨损的渣浆泵过流件，采用负压铸渗工艺成功制备了碳化钨颗粒增强铁基表面复合材料，改善和优化了复合工艺、铸造工艺。 |
| 9. | The system of vpi equipment mainly includes vacuum chamber , heating unit , worktable with its drive unit , vacuum pumping unit , gas - charge unit and control unit , etc . this equipment would meet the need of al or cu matrix composites prepared by pressureless infiltration or vacuum infiltration in a high vacuum or special atmosphere
该设备主要出密封罐、加热系统、升降工作台、真空系统、充气系统、控制系统及密封罐水冷系统等组成。可以实现常见金属基复合材料在较高真空或特定气氛下的无压浸渗或真空浸渗制备工艺，并可实现试样的不同冷却方式凝固。 |
| 10. | The results show that , composite could be fabricated on the complicated surface by means of sodium silicate sand mold combination with vacuum infiltration process . when the substrate was 10 mm , composite layers reached 3 mm to 5 mm in thickness with high strength interface and less casting defect . microstructures of grey cast iron and low chromium cast iron matrix composites with various volume fraction has been analyzed
结果表明：用水玻璃砂型(芯)加负压铸渗的工艺能够实现复杂表面(曲面)颗粒增强复合材料的制备，浇注方式简单可靠，基材厚度在10mm时，复合层厚度能达到3 5mm ，且复合层铸造缺陷少，复合效果好，界面致密而且结合强度高。 |
