| 1. | P . pastoris x - - 33 / s . . / hbsag was expressed under the induction of 0
川bsag ，在0 5甲醇诱导下进行分泌型表达。 |
| 2. | The second part of our experiment is the construction of recombinant plasmid pezz18 - as
第二部分是as基因分泌型表达载体的构建。 |
| 3. | Spla is able to induce the intracellular ceramide 2 2 generation followed by programmed cell death
分泌型磷脂酶a能诱导细胞的程序化死亡，并伴随有细胞2内神经酰胺生成的增加。 |
| 4. | Conversely , the intracellular sphingomyelinase ( smase ) can greatly enhance the cell death inducing effects of spla 2
相反，鞘磷脂酶则能显著增强分泌型磷脂酶a对细胞的毒性作用。 |
| 5. | The presented study provided novel experimental evidences for understanding the mechanism of the biological effects of spla 2
本研究为更深入的了解分泌型磷脂酶a介导生物学效应时2所涉及的机制提供了新颖的实验证据。 |
| 6. | Itwas observed that sphingomyelin plays anegative regulatory role on the activity of spla 2 , resulting in a strong inhibition on spla 2 - induced cell death
我们观察到，鞘磷脂对分泌型磷脂酶a的活性起着负2调控作用，它能有效的抑制分泌型磷脂酶a2对细胞的杀伤作用。 |
| 7. | By genetic engineering methods , ureb gene and ureb - hspa fusion gene were amplified by pcr and cloned into a prokaryotic expression plasmid ptrc99a - asd , and identified recombinant plasmid was then
口服、鼻饲免疫balb / c小鼠，在肠液和血清中可以分别检测到针对hpylori的特异性分泌型iga和址g抗体。 |
| 8. | The expression vector ppiczaa with the poifn - a gene was constructed by restrict enzyme digestion and ligation , then transformed into e . coli jm109 in order to extract recombinant plasmid from jm109
将此基因与毕赤酵母分泌型表达载体ppicz a连接，构建ppicz a - ifn重组质粒，并用常规cacl _ 2法转化大肠杆菌jm109进行扩增。 |
| 9. | In this thesis , the hplap gene was cloned into the ppiczaa plasmid and induced expressed in the protease - deficient strain p . pastoris smd1 168 . the clone that could secrete hplap with enzyme activity was selected
本研究将hplap基冈兑隆到spiczoa质粒中并在毕赤酵母尸pastorjs蛋白酶缺陷菌株smdi168中诱导表达，获得了有活性的分泌型hplap 。 |
| 10. | To study the biologic effects and the underlying mechanism of secretory phospholipase a 2 ( spla 2 ) and ceramide , we firstly investigated the relationship between certain lipid signalingmolecules - mediated signal transduction and the spla - induced cell death
为了研究分泌型磷脂酶a2和神经酰胺对细胞产生的生物学效应及其机制，我们首先探讨了某些脂类活性分子介导的信号传导与分泌型磷脂酶a诱导的细2胞死亡之间的关系。 |
