English translation for "杂交筛选"

screening by hybridization

Related Translations:

药物筛选法:  drug screening method

杂交植物:  tangelo

杂交配合:  xenogamy

杂交形式:  form of cross

杂交后代:  filial generation

盆栽杂交:  pot crossing

近亲杂交:  close crossingclosed crossing

变种杂交:  intervarieted cross

果实杂交:  fruit crossing

特定杂交:  specific cross

Example Sentences:

	1.	In this paper , we constructed the genomic dna library of nephila clavipes with the vector supercos 1 cosmid 以dig - oligo2为探针，菌落原位杂交筛选cosmid文库，得到56个阳性重组子。
	2.	Nor protein partner interacting with pgl29 was detected by yeast two - hybrid screening . thus further work needs to be made to understand the fu 酵母双杂交筛选结果，也未得到特异地与pgl29相互作用的直白质分于，因而现有的实验无法确定该基因的功能。
	3.	In our study , the igii subdomain of trka extracellular domain was fused to pgbkt7 as a bait to screen yeast random peptide library which contains 1 107 independent random peptide clones 我们将trk受体膜外域1911结构域与gal4蛋白融合构建成pgbkt7一ign ，确认无自激活作用后，将其作为诱饵蛋白，洲于酵母双杂交筛选。
	4.	Selected one of the 14 strains - s93 , s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18 , then transformed into dh5 a . filtering the clone with hybridization in situ , a 1 kb frament clone has been cloned 使用sau3ai对基因组dna进行不完全酶切，回收2 3kb片段，与puc18质粒连接转化大肠杆菌，利用地高辛标记探针，使用菌落原位杂交筛选转化子；筛选到包含有约1kb外源片段的转化子。
	5.	Four c - ch3 , six aromatic carbons , six carbons of mycosamine were the characteristics of candicidin d , one ketal corresponding to mycosamine and four ketons indicated that no hemiketal formed between c - 15 and c - 19 in fr - g08b or candicidin d . however , such hemiketal was usually thought having been formed 用这个探针，对链霉菌fr - 008的基因文库进行杂交筛选，获得了3个阳性克隆，经southern杂交分析，发现它们均含有6 . 4kb共同的阳性片断。
	6.	Correct clones were selected and plasmid dna was isolated and digested with saci and puvii . a dna fragment of about 2 . 1kb was purified and labeled by dig - 11dutp as probe . at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe . among them one clone contains human serum album dna by sequence 以pcr扩增的人血清白蛋白( hsa )基因片段为探针，从人的基因组文库中杂交筛选的阳性克隆中，经测序分析，有一个克隆含有全长hsadna ，用从其它的阳性克隆中选取两种dna片段，即dna修复基因hfen1和一段非编码大片段cit987sk - 384d8 ，与人hsadna一起，进行显微共注射，成功制备了转多基因小鼠。
	7.	Pit13 , an interactor binding to trpt1 protein , was screened by yeast two - hybrid , and confirmed by pull down and co - ip assays . the association of trpt1 with pit 13 demonstrated that trpt1 should probably participate in other activities besides in pre - trna splicing . more experiments will be required to determine the role of pit 13 protein 0酵母以杂交筛选、加卫工加wn检测及ip实验均证明m卜1 ”且与功能未知的pit13蛋； ”之间存在着相互作用，说明imj基因除了参与trna剪接之外，还可能具有其它方面的功能，有待进一步研究。
	8.	Positive probe was made from the floral meristem materials cultured for 5 days and 10 days with hormones by rna reverse transcription and labelling with radioactive dctp ; two negative probes from uncultured explants and it cultured for 5 days and 10 days . then , we did calculating signal arrays , sequencing , sequence analysis and alignments on genebank etc . finally 229 different ests were got from the cdna library 用在含有外源激素的培养基上培养5天(花分生组织开始形成)和10天(花分生组织已基本形成)的风信子外植体为材料，构建起cdna文库。利用cdnamicroarrray技术，经过杂交筛选和est分析序列分析，最终从cdna文库中获得了229个不同的外源激素诱导响应的基因序列。

Similar Words：

"杂交品种" English translation, "杂交亲本" English translation, "杂交亲和性" English translation, "杂交群" English translation, "杂交弱势" English translation, "杂交生物合成" English translation, "杂交石竹北极星系列" English translation, "杂交实生苗" English translation, "杂交试验" English translation, "杂交试验用薄膜过滤器" English translation