| 1. | 2 ) the rat - mouse reconstructed embryos activated were been cultured in the maturation mediums of tcm199 , ml6 , czb and czbm ( czb modified ) for 48h
直接注射法和融合法构建的重组胚桑椹胚发育率分别可达9 . 84 、 9 . 48 。 |
| 2. | When the temperature was at 25 , the efs30 ops two - step method had a blastocyst rate of 62 . 22 % , the highest rate in all groups
改用ops二步法efs30冷冻组保存后的2 -细胞胚胎的囊胚发育率高达62 . 22 ，为试验中的最佳组。 |
| 3. | The fresh mouse morulae were bisected following decompaction ( experiment 3 ) and other morulae without decompaction ( experiment 4 ) also splitted
新鲜桑椹胚经去致密化处理后分割，其分割成功率、半胚培养后的囊胚发育率都显著高于未处理组。 |
| 4. | Superovulation was induced by injection intramuscular of different dosages of fsh once daily for 5 days or fsh + 30 % pvp once , and then injection intravenous of 100 iu hcg on the 6th day
经血清饥饿3d 、 4d 、 sd同步化处理构建的重组胚，其桑堪胚发育率分别为20 22 、 18 |
| 5. | There were no significant difference in fusion rate , cleavage rate and blastocyte development rate with different enucleation time of oocytes during ivm16 - 24h ( p > 0 . 05 )
在体外成熟16 24h期间，水牛卵母细胞去核时间的不同对核移植后的融合率，分裂率和囊胚发育率没有显著影响。 |
| 6. | The results show that the development rates of morula treated with serum starvation for 3d , 4d , 5d are higher apparently than nocodazole group and control group ( p < 0 . 05 )
直接注射法构建的核移植重组胚胎的桑堪胚发育率（ 25 95 ）高于电融合法（ 16 07 ） ，统计学分析差异显著（ p 0 |
| 7. | External diameter of holding pipette is 130 ~ 150um , and internal diameter of injection pipette is 25um . hi this experiment , 311 embryos was obtained . in vitro cultivation developmental rate of 26 microinjected embryos is 65 . 4 % ( 17 / 26 )
在本试验中，获得胚胎311枚， 26枚注射体外培养发育率为65 . 4 % ( 17 / 26 ) ，将164枚注射胚和121枚未注射胚混合移植到14头受体。 |
| 8. | The enucleated rates can reach at 87 . 06 % . 3 ) three activated methods may effectively activate the black bear - cat reconstructed embryos . whereas activated effect of ethanol + 6 - dmap + ccb is more effective , the cleavage rate and the development rate to m
重组胚在3种培养液中的发育能力有差异t 0刀引， m199成熟培养液比dmem12 、 m16更能支待重组胚胎的卵裂和桑棋胚发育，卵裂率和桑堪胚发育率分别为63 |
| 9. | In the 2 - step ops method , embryos were pretreated with 10 % eg + 10 % dmso for 30s before exposed to edfs30 , a vitrification solution , for 25s , then immersed in liquid nitrogen . the blastocyst rate of vitrified mouse morula after equilibration in 0 . 5mol / l sucrose for 5min was 100 %
Ops二步法即胚胎预处理30s ，然后移入edfs30冷冻液中平衡25s冷冻保存，解冻后在0 . 5mol l蔗糖溶液中平衡5min ，培养后囊胚发育率为( 100 ) 。 |
| 10. | According to the mechanism of block of development in vitro culture on early embryo of mammal and in vivo surroundings of early embryo , the paper states that requirement and utilization of nutrients during each cell stage of early embryo of mammal in vitro culture in order to search for in vitro culture condition and method to improve the development rate of blastosphere
摘要从哺乳动物早期胚胎体外培养发育阻断机理和早期胚胎的体内环境入手，阐述了胚胎体外培养各细胞阶段胚胎对营养物质的需求，寻求合理的体外培养条件和方法，以便提高体外胚胎早期的囊胚发育率。 |
