| 1. | There were two plasmids in the isolated lactobacillus plantarum
分离的受体菌植物乳杆菌中含有两种质粒。 |
| 2. | The highest of transformants could reach 107 with per ng dna in bmb171
78 11等3种常用受体菌相应最高转化频率的2 ~ 3500倍。 |
| 3. | Result of sds - page analysis indicated that the expression of the protein was strong in the receptor
Sds - page分析表明该基因在受体菌hd73cry ~ -中能正常表达133kda蛋白。 |
| 4. | Sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored little ability of nisin production
以lactococcuslactisnz9800为受体菌，研究了表达载体的表达情况。 |
| 5. | Two main parts were included in the present thesis . part i . cloning and expression cryigenes of bacillus thuringiensis in different host strains
一、苏云金芽胞杆菌杀虫晶体蛋白基因在不同受体菌中的表达1 |
| 6. | All plasmids bearing icps genes introduced in bmb171 could produce characteristic insecticidal crystal proteins through expression of relevant icps genes
78 11 ，而表达cry1ca和cry3aa的表达量高于这3种对照受体菌。 |
| 7. | Gfp can be expressed in the host enterococcus facecalis and lactobacillus plantarum we isolated using the expression vectors we constructed the expression level depends on the nisin concentration to some extent
Ow洲poi114no例烃们表达载体在我们分离到的受体菌efqecalis和l plantarum中能够表达绿色荧光蛋白oppx表达的水平依赖于一定范围浓度的nistn诱导。 |
| 8. | After screening the relevant bacillus thuringiensis recipients and studied the optimized parameters of high - frequency transformation by electroporation , this ph . d dissertation established a high - frequency transformation system for bacillus thuringiensis
本研究通过筛选苏云金芽胞杆菌受体菌和对受体菌电脉冲转化条件的研究，建立了一种苏云金芽胞杆菌的高频转化系统。 |
| 9. | Sds - page analysis of the expression products indicated that the recombinant e . coli bl21 ( de3 ) plyss strain expressed an additional protein ( comparing with the control ) . the molecular mass of the protein equaled to the theoretically deduced value
表达产物经sds - page可见重组质粒受体菌的空白质粒受体菌多表达了一种蛋白，且这个蛋白质的分子量与理论推导值相符合，表达含量为11 . 80 。 |
| 10. | A gene library of psedomonas fluorescens g2 was constructed in the cosmid vector pla2917 using e . coli jm109 as the host strain . two recombinants , pgr3 and pgr7 , which can confer glyphosate resistance ofe . coli jm109 were identified from the selective medium containing 10mm glyphosate
以粘粒pla2917为载体、大肠杆菌jm109为受体菌构建荧光假单胞菌g2的基因组文库，在含有10mm草甘膦的固体选择培养基上筛选出两个耐受克隆pgr3和pgr7 ，插入片段分别为7kb和11kb 。 |
