Chinese translation for "可变区基因"
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- v-gene
Related Translations:
可变: variable; changeable◇可变比 variable ratio; 可变成本 direct costs; variable costs; 可变电容 variable capacity; variable capacitance; 可变电容器 variable condenser; variable capacitor; variodenser; adjusta 可变点: running pointvariable point 可变减速: varial deceleration 可变翼: variable geometry; swing-wing◇可变翼飞机 adjustable [variable] wing plane; swing-wing
- Example Sentences:
| 1. | Cloning and expression of the variable region genes of the monoclonal antibody against human cd
16单克隆抗体可变区基因的克隆和表达 | | 2. | Cloning of variable region and signal peptide genes of anti - cd20 monoclonal antibody by rlm - race
20单克隆抗体可变区基因及其信号肽基因 | | 3. | Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library . the products were cloned into puc19 vector and their sequences were analysed . the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region , with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以从噬菌体抗体库中筛选获得的抗hbsag的fab抗体基因为模板，分别扩增出其轻、重链可变区( v _ l 、 v _ h )基因，通过重组pcr方法将轻、重链可变区基因用连接肽( gly _ 4ser ) _ 3的编码序列连接，并引入前导肽编码序列，构建具有l - v _ h - linker - v _ l结构的单链抗体基因。 | | 4. | Methods : the rearranged gene fragment coding tcr y v region of the jurkat cell line was obtained by rt - pcr technique the pcr product was cloned into the eukaryocytic expressive vector pcdnas to construct pcdna3 / tcr y . after confirmed by sequncing . pcdnas / tcr y plasmids were amplified in bacteria extracted by alkaline lysismethod
方法：本文采用rt ? ? pcr的方法扩增jurkatt淋巴瘤细胞特异性重排的tcr可变区基因片段，克隆到真表达载体pcdna _ 3中，经序列测定无误后，碱裂解法大量提取质粒，制备dna疫苗。 | | 5. | In the present study , the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis . total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies . after obtained using rpas system , vh and vl genes were used to assemble scfv gene fragment with a linker primer
应用重组噬菌体抗体库技术，从分泌小鼠抗牛精子sp18抗体的杂交瘤细胞系中分离总rna ，克隆抗体重链和轻链可变区基因，加入连接肽引物( linkerprimer )组装成单链抗体scfv ( singlechainfragmentvariable )基因并用rs引物进行扩增， sfi 、 not酶切，回收后与pcantab5e载体相连，转化e . colitg1宿主菌，构建单链抗体文库。 | | 6. | However , dna damage has been ruled out as the prerequisite for alkylating agents - induced mutagenesis . for example , somatic hypermutation , which occurred around the variable region in the immunoglobin gene of b cells , is driven by antigen activation not by dna damage and is considered a kind of active mutation
但是dna损伤并不是引起突变的必要条件，一个典型的例子就是发生在b淋巴细胞的免疫球蛋白( ig )可变区基因上的“体细胞超突变( somatichyermutation ) ” ，是由表面抗原受体而不是dna损伤驱动的主动突变。 | | 7. | Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重组噬菌体抗体库的构建及鉴定从培养的mg _ 7杂交瘤细胞中提取并分离mrna ，反转录成cdna ；利用pcr分别扩增mg _ 7单抗的重链及轻链可变区基因，并通过? dna连接子将二者连接起来形成mg _ 7单链抗体基因；将mg _ 7单链抗体基因插入pcantab5e ；将连接产物转化感受态tg1大肠杆菌，制备细菌形式的mg _ 7重组噬菌体抗体库；通过菌落计数和限制性酶切分析( ecor和hind )评估mg _ 7重组噬菌体抗体库的容量和重组率。 |
- Similar Words：
- "可变青霉菌" Chinese translation, "可变倾角" Chinese translation, "可变倾角式喷燃器" Chinese translation, "可变情报" Chinese translation, "可变区" Chinese translation, "可变区取样" Chinese translation, "可变区群" Chinese translation, "可变区亚群" Chinese translation, "可变区域" Chinese translation, "可变区域；变面积" Chinese translation
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