5s rdna cloning and sequencing were carried out to analyze the 5s rdna variation pattern . genomic in situ hybridization ( gish ) was performed for better understanding of genomic relationship among closely relative pines 利用克隆测序方法对松属植物5srdna的研究无疑是有开创性的工作，可以探讨裸子植物的5srdna的进化机制和种间关系。
