This material was loaded onto a silica gel column and the column was eluted with chloroform several times until the fraction of interest was collected . finally we obtained some light yellow green ointment liquid f i 采用硅胶作干层析柱填充物，用氯仿作展开剂和洗脱液，反复硅胶层析，对柄海鞘鞘囊氯仿浸提物进行分离、提取与纯化，最后得到一浅黄绿色的油状液体fraction (以下均称f ) 。
