Chinese translation for "细胞化学方法"

cytochemical methods

Example Sentences:

	1.	Cells were centrifuged for 5min at 1000rpm and removed supernatant . the cell peller was resuspended in 1 ml culture media and divided into two glass flask . cell surface makers ( cd45 , cd90 ) were detected by immunocy - tochemistry 当传到第五代时，取一瓶mscs用胰酶消化，以8x10vcm ‘浓度接种在六孔板中的盖玻片上，应用免疫细胞化学方法检测cd45人d90的表达。
	2.	The fetal liver stem cells were isolated by collagenase digestion , gravity sedimentation and density gradient centrifugation , identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition 采用胶原酶消化、重力沉降及密度梯度离心方法分离人胎肝干细胞，通过免疫细胞化学方法对其进行初步鉴定，以及应用流式细胞仪等对其生长状况进行评估。
	3.	In view of its high economic values , the author studied the ultrastructure of the mature sperm and spermatogenesis of cipangopaludina chinensis in order to supply the basic materials of the reproductive biology of cipangopaludina chinensis 鉴于其较高的经济价值，作者运用光镜、电镜和细胞化学方法，对中国圆田螺成熟精子和精子发生的超微结构进行研究，以期为中国圆田螺的繁殖生物学研究提供基础资料。
	4.	Is important for implantation and decidualization . the aim of this study was to examine the expression and regulation of ep2 gene in rat uterus during early pregnancy , and under delayed implantation and artificial decidualization by in situ hybridization and immunohistochemistry 本实验利用原位杂交和免疫细胞化学方法，检测了ep2基因在大鼠早期妊娠子宫中的表达，并利用延迟着床和人工蜕膜化模型研究了ep _ 2基因在子宫中的表达与调节。
	5.	M ethods we successfully expanded human embryonic brain - derived nsc into spheres with mitogens . the nsc were identified by immunocytochemistry method , brdu labeling and cell cloning were used to observe the proliferation ability of nsc . pdgf x t3 were separately used to induce the differetiation of nsc 方法：本实验以胎龄为10 - 12周的人大脑皮质为材料，在体外成功诱导扩增nsc ，用免疫细胞化学方法鉴定nsc ，用brdu标记和细胞克隆分析观察了nsc的增殖能力。
	6.	We detected the transcription and translation change of different thrs genes by semiquantitative reverse transcription - polymerse chain reaction ( rt - pcr ) analysisjelisa respectively . at same time the differentiated cell were identified by immunocytochemistry method . results ( l ) nsc induced by mitogens were nestin - positive and incorporated by brdu 以pdgf 、 t _ 3对nsc进行诱导分化，并分别用rt - pcr半定量法、 elisa法检测nsc在不同诱导因子作用下分化前后thr各亚型在转录与翻译水平上的表达变化，用免疫细胞化学方法鉴定分化后的细胞类型。
	7.	Injected group , 0 . 1 % saccharin ( 1 . 5 ~ 2ml / rat , in 5min ) intraoral infused group and cta group . the expression of endogenous leucin - enkephalin ( lek ) in the rat brain was observed and 5 parts of the thalamus including laterodorsal thalamic nucleus ( ld ) , lateral part of mediodorsal thalamic nucleus ( mdl ) , ventroposterolateral thalamic nucleus ( vpl ) , ventroposteromedial thalamic nucleus ( vpm ) and reticular thalamic nucleus ( rt ) were comparatively researched before and after the acquisition of cta applying lek - immunocytochemistry . in behavioral experiment , 18 adult male sd rats were divided into normal cta group ( control ) and 2 naloxone i . p 为探讨cta形成过程中enk的作用，本实验用成年雄性sd大鼠35只，分为空白对照组、生理盐水( 2体重)腹腔注射组、 0 . 15mlicl溶液( 2体重)腹腔注射组、 0 . 1糖精溶液口腔灌流组( 1 . 5 - 2ml只， 5min )和cta建立组，采用免疫细胞化学方法，观察了亮脑啡肽( lek )阳性神经元在大鼠脑内的分布情况，并比较了各组大鼠丘脑外侧背核( ld ) 、丘脑内侧背核外侧部( mdl ) 、丘脑腹后外侧核( vpl ) 、丘脑腹后内侧核( vpm )以及丘脑网状核( rt )等5个脑区内lek表达水平的差异；另外将成年雄性sd大鼠18只，分为正常cta建立组以及在cta建立前或cta建立后阿片受体拮抗剂纳洛酮( 2mg kg体重)腹腔注射组，对内源性阿片样物质对于cta建立和保持的影响进行了行为学研究。

Similar Words：

"细胞化学" Chinese translation, "细胞化学超微结构" Chinese translation, "细胞化学成份" Chinese translation, "细胞化学的" Chinese translation, "细胞化学反应" Chinese translation, "细胞化学活性" Chinese translation, "细胞化学检验" Chinese translation, "细胞化学染色" Chinese translation, "细胞化学生物测定" Chinese translation, "细胞化学性质" Chinese translation