We also stained the embryos with anti - arp3 antibody and found that these two kinds of antigen are generally co - localized but arp3 were not detected in cleavage stage embryos . it was deduced that cortactin functions in the cytokinesis independent of arp3 . and microinjection of anti - cortactin antibodies into the i cell stage embryos leaded to fatal developmental defects because of the unequal cytokinesis 在利用抗ap3的抗体进行的胚胎整装染色中，激光扫描共聚焦显微镜观察发现， arp3在卵裂期没有表达， mbt后，在细胞皮层区检测到arp3蛋白的阳性染色，在随后的发育过程中arp3与皮层蛋白共分布。
