| 1. | The measured complexity of the cdna library is 4 . 134x 10s directional clones
分析结果表明，该未扩增的cdna文库大约含有4 . 134xlo ，个克隆。 |
| 2. | Pcr products amplified from an elicitor induced cdna library were sequenced and analyzed
以稻瘟病菌的细胞壁成分诱导的cdna文库为模板，对cdna进行克降。 |
| 3. | 3 . il - 6r gene was amplified by pcr from the brain cdna library of the normal adult rat
三、用pcr技术，从正常成年大鼠脑的cdna文库中，获得编码il6r基因的序列。 |
| 4. | Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e . coli strain dh5 - alpha to generate cdna library that size is 4 . 9 l06 recombinants
将cdna与载体连接，并导入dh5感受态细胞中，构建成cdna文库。 |
| 5. | The library size is 1 . 2x 106 recombinants . the average length of inserted fragments in the library was longer than 450bp , which was testified by both pcr and sequencing analysis
蓝白斑检验结果表明cdna文库滴度为1 . 2 10 ~ 6 ，重组效率为95 . 9 ， pcr检测及序列分析结果验证插入片段平均长度大于450bp 。 |
| 6. | Rbp - j directly interacts with ramic to activate transcription of downstream target genes that prevent cell differentiation . thus , rbp - j is a critical effector molecule in notch signaling pathway
5dpc胚胎和人的hela细胞cdna文库中筛选到一个可以与rbp - j第四军医大学博士学位论文相互作用的新蛋白kyot |
| 7. | Because the sequence and functions of every chromosome of c . elegans have been studied completely . so , first we employed the rapgap and cdna library from celegans to start the screen
由于对c . elegans的研究较详细，对其每个基因的功能及dna序列已经清楚，故我们首先应用cons来源的rcygap作为饵蛋白探查celegqns的cdna文库。 |
| 8. | In the third part , we used m4g3 ascites as probe to screen the library for obtaining positive clone . once it was found , we would go on sequencing and analyzing at web ncbi / blast
第三部分：应用m4g3单克隆抗体筛选cdna文库，得到阳性克隆，并加以纯化，序列分析，通过internet互联网络使用ncbi blast程序库进行同源性检索。 |
| 9. | We detailed here a novel gene clsp , isolated from the dc cdna library by large - scale random sequencing . full length of clsp contains 3345 bp , with an orf of 1464 bp
经大规模随机测序和同源比较，我们从人树突状细胞cdna文库中分离到一个新表达序列标签( est ) ，其全长cdna为3345bp ，含有1464bp的开放读框，编码含487个氨基酸的蛋白。 |
