| 1. | The recombi - nant plasmid pgex - hnadc3 was identified by dma sequencing and restriction enzyme mapping
限制性内切酶酶切及dna测序鉴定重组质粒。 |
| 2. | The accuracy and stability of the typing result typing results match the set results , and in 5 hybridization , the repeated rate is 91 . 2 %
3寡核营酸芯片分型结果的准确性与稳定性分型结果与dna测序相符合，重复5次杂交重现率91 . 2 % 。 |
| 3. | The dna sequencing verified that the pgex - hnadc3 contain ! - ed 482 - 697bp encoding 155 - 226 aa of the hnadcs with correct open reading frame
Dna测序证实重组质粒pgex十nadc3含有hnadc3基因的第482 ? 697位核昔酸序列，编码155 226位氨基酸，读码框架正确。 |
| 4. | Pcr products were purified with qiaquick pcr purification kit , followed by sequencing using the big dye terminator cycle sequencing ready reaction kits used with amplitaq dna polymerase fs
以pcr纯化试剂盒纯化pcr产物，然后进行测序反应。测序在在dna测序仪( 377 )上进行。 |
| 5. | Pcr products were purified with qiaquick pcr purification kit , followed by sequencing using the big dye terminator cycle sequencing ready reaction kits used with amplitaq dna polymerase fs
Pcr产物用pgr纯化试剂盒纯化之后，进行测序反应。最后标本在dna测序仪( 377 )电泳和分析。 |
| 6. | The 5 - copy gene was expressed when induced in different hosts . sds - page analysis showed three recombinant plasimids all produced induced expression bands that accord with expected
Dna测序正确后，转化不同的宿主菌中进行诱导表达， sds - page分析发现三种重组质粒在对应位置均出现特异的蛋白表达带。 |
| 7. | Proper multi - copy gene was selected and cloned into puc19 vector . restriction enzyme analysis and dna sequencing confirmed that 5 - copy gene was correctly inserted into the vector
选取合适拷贝数的串连重复基因，将其克隆至puc19载体，双酶切、 pcr扩增和dna测序证明串连重复基因构建成功且基因方向相同。 |
| 8. | Particularly risky experiments are underway , he says , yet who experts have agreed that no valid reason exists to retain smallpox virus stocks for dna sequencing , diagnostic tests , or vaccine development
极其危险的试验正在进行,他说,但who的专家们一致认为,没有为dna测序,诊断试验或疫苗研制而保留天花病毒储存的正当理由 |
| 9. | Thirdly , this new plasmid is identified by double - enzyme cutting . ncoi - cutting . pcr by different groups of primers and dna sequencing , the results show the new plasmid ppic9k - ple , that is expression plasmid , is constructed successfully
该质粒经双酶切、不同引物的pcr扩增、 nco酶切等方法鉴定，以及dna测序，证明ppic9k - ple表达载体构建成功。 |
| 10. | Cloning and expression in e . coli of lactase . specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis . klac gene was amplified by pcr and subsequently cloned
乳糖酶基因的克隆及原核表达以乳酸克鲁维斯酵母( kluyveromyceslactis )菌株k538的基因组dna为模板，设计引物，利用pcr获得乳糖酶基因klac ，经dna测序验证，得到克隆t1549 。 |
