Chinese translation for "echistatin"

锯鳞血抑

Example Sentences:

	1.	Conclusion constructed the high - level expression clone of echistatin in e . coli . the expression of recombinant protein is higher , it make the further study of echistatin feasible 结论成功构建了echistatin的原核高效表达克隆，表达量高于现有国内外研究水平，为echistatin功能及相关疾病的研究奠定了基础。
	2.	Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing 目的构建蛇毒锯鳞蝰素( echistatin )的原核高效表达体系方法由genebank数据库检索蛇毒锯鳞蝰素( echistatin )的氨基酸序列，结合大肠杆菌蛋白质合成体系对氨基酸密码子使用的偏爱性，设计了echistatin编码基因，体外人工合成编码基因dna片段，通过适当的限制性内切酶位点插入表达载体pbv220 ，分别构建了echistatin的单拷贝表达克隆、双拷贝串联表达克隆；进一步通过pcr技术构建echistatin的融合表达基因克隆。
	3.	Results : designed and synthesized the coding gene of echistatin . and constructe its single copy expression vector , identify the vector by dna sequencing . but it could not express echistatin in e . coli . designed and synthesized the modified coding dna of echistatin , and constructe its two copy expression vector , identify the vector by dna sequencing , but it could not express echistatin in e . coli 结果设计并合成了echistatin编码基因dna序列，构建了echistatin单拷贝表达载体，经dna测序鉴定正确后， sds - page及western - blotting结果表明： echistatin在上述菌株中未获得高效表达。
	4.	Constructed the fusion protein expression vector of echistatin , . and identify it by dna sequencing . the vector can express echistatin fusion protein at a high level , the fusion protein molecular weight is about 16000 da in sds - page analysis , and the fusion protein consists more than 30 % of total protein , the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody . the fusion protein include : hpk5 . his + 6tag , and echistatin . echistatin can be released by cnbr cleavage 构建了echistatin融合蛋白表达载体，经dna测序鉴定正确后，温控诱导表达，获得了高效表达，表达产物经505一队ge分析，分子量为16000oa ，符合预计的融合蛋白分子量，表达产物. ll ’菌体总蛋白的30 %以上， westem一blotting结果显示，该日的蛋白能够和echistatin多克隆抗体发生特异性抗原抗体反应，表明我们成功构建了eohistatin的高效融合表达克隆。

Similar Words：

"echioidinin" Chinese translation, "echira" Chinese translation, "echire" Chinese translation, "echiretin" Chinese translation, "echis carinatus" Chinese translation, "echistov" Chinese translation, "echiststin" Chinese translation, "echitamine" Chinese translation, "echitenin" Chinese translation, "echitenine" Chinese translation