| 1. | Gel filtration chromatography , gfc
凝胶过滤色谱法 |
| 2. | Gel filtration chromatography
凝胶过滤色谱法 |
| 3. | The results of superose 6 gel - filtration chromatography and western blot suggest that the deletion mutant cause a gigantic change in global structure of arog
第三部分工作研究了户四g结构上的“ dz ”对称性在反馈抑制中的作用。 |
| 4. | The purified badh , which became a single band in sds - page , has been obtained by a combination of ammonium sulfate fractionation , ion exchange chromatography and gel filtration chromatography
辽宁碱蓬甜菜碱醛脱氢酶分子量为129kd ，单个亚基的分子量为64 . 5kd ，表明其为同型二聚体。 |
| 5. | It was very slow at 5 but become higher above 25 . the enzyme from abomasums used in cheese production was rough extracts containing chymosin and pepsin . through the gel filtration chromatography and ion exchange chromatogra
羔羊胃蛋白酶最适凝乳温度为45 ’ c ；在45处理30inin ，酶活性开始下降， 60c处理30lliln ，酶活性完全丧失；酶的最适ph为1 |
| 6. | The separation of the formica rufa linnaeus samples by pel filtration chromatography . in this part , the author has primarily separated the formica rufa linnaeus samples by glucosan gel g - 50 filtration chromatography . the principle is the difference of the molecular weight of different components
红褐林蚁样品的凝胶过滤层析分离本部分旨在通过葡聚糖凝胶g - 50过滤层析初步分离红褐林蚁样品，根据分子量大小不同而达到分离目的。 |
| 7. | One is a combination of ion exchange chromatography on q sepharose ff , hydrophobic interaction chromatography on phenyl hp , gel filtration chromatography on sephadex 200 and higher resolution ion exchange chromatography on mono q . the other is a combination of ion exchange chromatography on q sepharose ff , two affinity chromatography on con a sepharose 4b and benzamidine sepharose 6bcl sequentially
上清液中重组蝗蛇毒类激血酶的纯化是通过设计的不同分离纯化方法组合进行的，并对每一种组合进行了活力与纯度及口收率的评价，结果表明两种组合方式都具有一定的实用性和可操作性。 |
| 8. | The antimicrobial secretion could be divided into three sections , every section had antimicrobial activity . the first could be extracted by petroleum ether , the second could be extracted by ethyl alcohol , the third could dissove in water and could be separated by sephadex g - 50 , g200 gel filtration chromatography and carbornmethyl cellulose - 1 anion - exchange chromatography and detected by a256 , . the antimicrobial secretion had wide spectrum and had strong inhitory activity against germs and fungi , they could inhibit sixteen kinds of plant pathogenic germs , eight kinds of animal germs and eight kinds of plant pathogenic fungi
粗提物经石油醚萃取可得第一个活性部分，剩余部分经无水乙醇萃取可得第二个活性部分，剩余物质再经凝胶sephadexg - 50后有两个峰，第二峰有活性，再经凝胶sephadexg - 200后有三峰，第二峰有活性，将其经过阳离子交换柱cm - 1后有两峰，此两峰均有抑菌活性。 |
| 9. | Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease . serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography . the t profile , t stability profile , ph profile , substrate specificity and inhibitor were tested
该酶分子n -端的氨基酸序列为avidtgveashpef ，通过n -端氨基酸序列设计简并引物，提取被毛孢owvt - 1的总rna ， rt - pcr克隆了该酶的成熟肽编码基因( 1000bp ) ，序列分析表明，丝氨酸含量高达12以上。 |
| 10. | Strain bl21 , and gene expression was induced by iptg . the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene . using ion exchange chromatography and gel filtration chromatography , the chimera proteins were purified , and both of them showed a single band in tricine - sds - page . the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities , but gain platelet aggregation inhibitory activities as well
通过离子交换层析和凝胶过滤层分别对两种嵌合体蛋白进行纯化，纯化产物在tricine - sds - page中都显示为单一条带。活性分析结果表明两种嵌合体蛋白在保留水蛭素抗凝血酶活力的同时，还呈现抗血小板聚集活性。 |
