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Home > english-chinese > "fusion expression vector" in Chinese

Chinese translation for "fusion expression vector"

融合表达载体

Related Translations:
expression equipment:  压榨设备216
ventricular fusion:  心室融合
interbody fusion:  椎体间融合术
commissural fusion:  连接点溶合
fusion repairing:  溶补
fusion bass:  融合贝斯熔化贝司
carporadial fusion:  腕桡关节融合术
fusion neutron:  聚变中子
fusion plasma:  聚变等离子体
arc fusion:  电弧熔接
Example Sentences:
1.So , we subcloned emt - 1 gene into the prokaryotic fusion expression vector prsetb and generated a 24ku protein
为此将emt l氨基末端基因克隆入融合表达载体prsetb中,转化大肠杆菌bl 21 ,经iptg诱导,表达his6 emt l融合蛋白,表达产物分子量约为24ku 。
2.Target gene was cloned into the procaryotic fusion expression vector pet28a ( + ) , then subcloned into the eucaryote expression vector pcdna3 . 1 ( + ) after sequence analysis
将halr基因克隆到原核融合表达载体pet28a ( + )上,序列分析后亚克隆到真核表达载体pcdan3 . 1 ( + )上。
3.Purification of the recombinant expressed endostatin in this work we have successfully cloned mouse endostatin gene , and constructed pbv220 - endostatin expression plasmid that is a non - fusion expression vector . the positive pbv220 - endostatin was transformed into dh5a , and expressed mouse endostatin protein successfully . this study is the basis of the endostatin gene - engineering production
本文成功克隆鼠内皮抑素基因,构建了内皮抑素基因的原核表达载体pbv220一endostatin ,该载体为非融合性表达载体,将pbv22o一endostatin成功转化到大肠杆菌表达菌dh5a中,并诱导表达了鼠内皮抑素蛋白,为基因工程方法生产内皮抑素奠定一定基础。
4.The total rna was extracted from human normal kidney tissue and the cdna fragment of hnadcs was produced by rt - pcr , then was purified and inserted into pgem - t vector . after dna sequenc - ing , pgem - hnadcs was digested with ecor i and sal i , and ? the dma fragment was subcloned into the ecor i and sal i sites of the fusion expression vector ( pgex - 5x - 1 )
从正常人肾组织中提取总rna , rtpcr扩增hnadc3抗原表位区cdna片段,产物克隆至pgem吓载体,测序正确后,将hnadc3抗原表位区dna片段再次亚克隆至pgex融合表达载体,构建重组质粒pgex十nadc3 。
5.The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others , especially by lixinlal in naigin normal university of china , we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence , using the favorable condon of the ecoli . after cheked the result of synthesis by sequence , we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ) , and the fusion protein of thio - cmiv was highly expressed in soluble form
本研究的内容包括:一、在前人对抗菌肽cmiv研究的基础上,对n端和c端进行氨基酸保守变换,设计和合成了该基因,充分使用大肠杆菌偏爱的密码子,并将该基因5端与硫氧还蛋白基因3端融合,通过ptxfus表达载体获得较高可溶性表达(在15 sds - page胶上可见明显的表达蛋白带) 。
Similar Words:
"fusion engineering research facility" Chinese translation, "fusion enthalpy" Chinese translation, "fusion entropy" Chinese translation, "fusion epiphysiodesis" Chinese translation, "fusion experiment" Chinese translation, "fusion face" Chinese translation, "fusion facility" Chinese translation, "fusion faculty" Chinese translation, "fusion faculty theory" Chinese translation, "fusion first wall" Chinese translation