The manufacture of thiamine from the simple substances present in the minimal medium does not take place in a single chemical reaction . 从最低培养基中的简单物质制造硫胺素，不是通过单一的化学反应。
2.
Compared with minimal medium , rich medium leads to higher expression level up to 4 mg target protein / l . the optimal culture conditions we - e obtained as follow5 : complex medium buffered to ph 6 . 0 ; harvesting cells after 36 hours of methanol induction 对菌体培养条件进行的探索表明：蛋白表达的最佳ph值应为6刀，最佳诱导时问为36小时，培养基为营养丰富的复合培养基。
