| 1. | However , the advent of new molecular techniques has dramatically changed the nature of chromosome mapping in all organisms
新的分子生物学的方法的出现改变了所有生物染色体图谱的本质。 |
| 2. | Meanwhile , molecular techniques are much better than traditional morphological methods in hybridization study
而在杂交研究领域，文章揭示了分子生物学手段有着比传统形态分析方法更多的优越性。 |
| 3. | Molecular techniques , such as plasmid profiles , have also been of limited value in identifying individual strains of vibrio cholerae
在个别霍乱弧菌的菌株?定上，分子生物技术如质体图谱的价值也是有限的。 |
| 4. | Using arious cell and molecular techniques , the researchers discoered that insulin stimulates human keratinocytes in culture to proliferate and migrate
通过各种细胞分子技术，研究者发现胰岛素对培养的人角质化细胞有促进增殖和扩散的作用。 |
| 5. | Using various cell and molecular techniques , the researchers discovered that insulin stimulates human keratinocytes in culture to proliferate and migrate
通过各种细胞分子技术，研究者发现胰岛素对培养的人角质化细胞有促进增殖和扩散的作用。 |
| 6. | More and more methods of studying the mechanism for the space mutation have appeared , developing from observation to cell and molecular techniques , and some great progresses have been made
对航天诱变机理的研究方法也逐渐增多，从单一的形态观察向细胞水平、分子水平发展，并且取得了一些长足的进展。 |
| 7. | The research areas focus on the relationships between animal survival , reproduction , population density , population distribution and its environments , endangered mechanism of rare and threaten animal , and conservation of wildlife diversity and its habitats using ecological or molecular techniques
利用宏观及分子生物学技术，开展亚热带滨海资源动物的生物多样性研究，解决资源动物的保护生物学及可持续利用等问题。 |
| 8. | The trend is refered to combine the evidence from several genes representing both nuclear and organella genomes with morphology , ontogeny and life history for systematic studies ; while studies at the population level by applying molecular techniques with high differenciation lead an important direction
指出同时应用能够代表核和细胞器基因组的多个基因结合形态、个体发育和生活史进行系统学分析是其必然发展趋势；而应用高分辨率的分子手段进行居群研究是一个重要的发展方向。 |
| 9. | Rapd ( random amplified polymorphic dna ) , which bases on the polymerase chain reaction ( pcr ) , is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms . the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna , and a small number of fragments will be amplified when the primer anneals on each strand over a length range . if sequence variation is present at the priming site , then a fragment may not be amplied , so the dna polymorphic can be detected
Rapd (随机扩增多态性dna )技术是二十世纪90年代发展起来的一项dna分子多态性检测技术，它建立于聚合酶链式反应( pcr )技术基础之上，利用随机合成的寡聚核苷酸序列为引物(一般为10个bp ) ，分别与dna的两条单链结合，在dna聚合酶的作用下，对基因组的特定区域进行pcr扩增，其电泳结果为不同大小和数目的dna谱带即rapd图谱，可反映基因组相应区域的dna多态性。 |
| 10. | Based on the previous studies , the research in this paper was carried out , mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province . molecular techniques , such as fish , pcr , dna cloning and sequencing etc . were used for this purpose . ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied , which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process
本论文在前人研究的基础上，开展了以下两个方面的工作： ( 1 )采用分子生物学技术荧光原位杂交( fish ) 、多聚酶链式反应( pcr ) 、 dna克隆和测序等对采自江苏省苏州市、东太湖、新沂河等6个底质样品进行了厌氧氨氧化菌和传统氨氧化菌的原位检测； ( 2 )探讨了以底质作为接种体进行厌氧氨氧化菌富集培养的可行性，为天然底质环境中厌氧氨氧化过程的强化，富营养化底质微生物修复的可行性提供一定的依据。 |
