The active substance is unshiped from colophony through acet 以8000rpm对发酵液离心收集菌体。
2.
This paper discribes the preliminary investigation on radiolysis behavior of methylhydrazine ( mmh ) , ? - hydroxylethylhydrazine ( heh ) and acet - hydroximic acid ( aha ) in co - 60 y field 对甲基肼( mmh ) 、 -羟基乙基肼( heh )及乙羟肟酸( aha )等在co - 60辐照场中的辐解行为进行了初步的定性或定量研究。
3.
After the acet is vaporized , the active substance in water is gotten . and which is vaporized at low temperature . then the crude active substance is purified by column chromatography on sephadex g - 75 . after a series of purifications again , we could get some white powder at last . though the active substance is diluted to50 g / ml , the activity is still checkeded - up through phyto phtnora casicileon . the purified active substance is insensitive to heat , resistant to chloroform 、 ethanol and the orhers . in addition , the active substance is sensitive to high ph ( 10 ~ 14 ) , but it is not sensitive to low ph ( 1 ~ 5 ) . furthermre , when the ph is made to low again , the activity of it ' s comes back 用蒸馏水对菌体稀释；加入适量吸附树脂在150rpm 、 28下振荡吸附4h ， 80 %的丙酮解吸，过滤解吸液得到活性物质的澄清溶液，旋转蒸发仪旋转蒸发去处丙酮，经sephadexg - 75分子筛层析得单一活性峰，收集峰值部分样品液经冷冻干燥得到淡褐色粉末，该活性物质用丙酮充分洗涤、甲醇-乙醚重结晶获得略带微黄的白色粉末，该活性物质50 g / ml仍可对苏云金芽孢杆菌hd - 1产生明显的抑制作用。
