English translation for "antigen-binding"
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- 抗原-结合的
Related Translations:
- Example Sentences:
| 1. | Cloning and expression of fragments of fibronectin to screen hepatitis b virus surface antigen - binding domains
纤连蛋白的分段表达及与乙肝表面抗原相互作用研究 | | 2. | After dissolving inclusion bodies , renaturing inclusion bodies and further purification by immobilized metal ion affinity chromatography ( imac ) , the fusion protein trx - scfv of electrophoretic purity was obtained , they still possessed antigen - binding activity
经变性复性，用固相金属离子亲和色谱法一步分离纯化了具有功能的单链抗体，在一升的发酵液中得到78mg的重组蛋白。 | | 3. | Detection of antigen - binding affinity of soluble mg7 scfv elisa was adopted to examine the antigen - binding affinity of soluble mg7 scfv ; competitive elisa was performed to test the inhibitory ratio of soluble mg7 scfv to the binding affinity of mg7 mab with its relevant antigen
El旧a拐成惴现2个克隆的可容牲mg7seem明显的抗原结合舌队其a分别为0 832和0 912 ，均高出附性对照（ 0 | | 4. | Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen , the positive dones possessing apparent inhibitory effect were singled out for later use
X阳性克到驶知烘扳原kbbjg ）浊对阳性克隆进行限制陇卧赐析（ uwi和hedlll ）鉴定三用竞争elisatoljmg7重组噬菌体抗体性克隆对mg7单扶与其相应抗原结合忙的喇率，从中j ） ed出对mg7单抗与期眩抗原结合有抑余j效应的克隆用于进一涉研究。 | | 5. | ( 4 ) in artificial pepsin digestive system with ph2 . 0 , due to the acid denaturation , the pigg was hydrolyzed wholly , in the system with ph3 . 0 and 4 . 0 , although not all entirely pigg could be detected , but the pigg antibody activities against e . coli still existed without changing , this indicates that the f ( ab " ) 2 with antigen - binding activity still remain complete during digesting process
( 4 )在ph2 . 0的人工胃液消化体系中，由于酸变性pigg全部水解；在ph3 . 0和ph4 . 0的人工胃液消化体系中，尽管检测不到所有的pigg ，但pigg仍可与e . coli特异性结合且凝集价未发生变化，表明在消化过程中具有抗原结合活性的f ( ab ) _ 2片段仍可完整地保留下来。 | | 6. | Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重组噬菌体抗体库的构建及鉴定从培养的mg _ 7杂交瘤细胞中提取并分离mrna ，反转录成cdna ；利用pcr分别扩增mg _ 7单抗的重链及轻链可变区基因，并通过? dna连接子将二者连接起来形成mg _ 7单链抗体基因；将mg _ 7单链抗体基因插入pcantab5e ；将连接产物转化感受态tg1大肠杆菌，制备细菌形式的mg _ 7重组噬菌体抗体库；通过菌落计数和限制性酶切分析( ecor和hind )评估mg _ 7重组噬菌体抗体库的容量和重组率。 |
- Similar Words：
- "antigen-antibody system" English translation, "antigen-antibody test" English translation, "antigen-antibody union" English translation, "antigen-antiglobulin reaction" English translation, "antigen-associated erythrocytes" English translation, "antigen-binding capacity" English translation, "antigen-binding fragment" English translation, "antigen-binding fragments" English translation, "antigen-binding lymphocytes" English translation, "antigen-binding myeloma protein" English translation
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