| 1. | It was also proved that the biosynthestic genes of apramycin was linked to the apramycin resistant gene in s . tenebrarius
Pcr实验证明基因重组菌株中接合转移质粒同源整合到黑暗链霉菌h6的染色体上。 |
| 2. | The gene recombinant strain no . 42 ca n ' t generate ampramycin , which indicated that the cloned gene is involved in apramycin biosynthesis in s . tenebrarius
通过接合转移的方法将质粒pzxb014导入黑暗链霉菌h6中，筛选基因发生重组的菌株。 |
| 3. | To study the biosynthetics genes of apramycin in streptomyces tenebrarius , the apramycin resistant gene was isolated by gunshot cloning firstly
为了研究黑暗链霉菌安普霉素生物合成基因，首先通过鸟枪克隆的方法克隆其安普霉素抗性基因。 |
| 4. | The ligation mixture was used to transform s . lividans tk24 protoplasts . six colonies capable of growing in the presence of apramycin were isolated
连接液转化标准宿主菌s . lividanstk24原生质体，最终得到6个可以在安普霉素平板上生长的阳性克隆。 |
| 5. | This result proved that uv treatment combined with tolerating apramycin or with tolerating streptomycin could be effective way to obtain high yield apramycin - producing strains
证明了uv处理复合耐受自身产物、 uv处理复合耐受链霉素能获得较好的筛选结果。 |
| 6. | Amino acid feeding experiments were also performed in this study in order to investigate the pathway , by which nitrogen incoperated into apramycin molecule , and the regulatory mechanism of nitrogen metabolism
通过氨基酸添加实验研究了安普霉素生物合成途径中的氮的来源及调节机制。 |
| 7. | The 2 . 0 - kb bamhl - sphl fragment , upstream fragment of the apramycin resistant gene , was sequenced . analysis of the nt sequence revealed that it contained an orf from nt positions 877 to 1995
抗性基因上游2 . 0kbbamhi - sphi片段测序表明在该片段中有一个完整的orf ，位于877 1995bp之间，编码373个氨基酸。 |
| 8. | Plasmid psyxl , containing a 12kb insert of s . tenebrarius dna , was isolated from one of six colonies . apramycin resistant gene was located in 1 . 5 - kb sphl - kpnl fragment of plasmid psyxl though further studies
阳性克隆中重组质粒psyx1含有12kb黑暗链霉菌的dna ，对12kb片段进一步分析将安普霉素抗性基因定位在1 . 5kbsphi - kpni片段上。 |
| 9. | The tertiary fermentation techniques and the fbc ( fed - batch culture ) and other fermentation techniques were also optimized . a scale - up fermentation technique was set up and the apramycin productivity reached 4478u / ml in a 4m3 fermentator
对三级发酵工艺、补料工艺等发酵工艺条件进行了优化，进一步确定了中试发酵工艺条件， 4m ~ 3发酵罐上平均发酵单位达4478u ml 。 |
| 10. | Streptomyces tenebrarius a04 , the producer ( with a titer of 2874u / ml ) of single component of nebramycin ? apramycin was studied in this paper . after treatment of spore suspension and mycelia shivered by supersonic with mutagen , combined with the application of screening models , some stable high yield apramycin - producing strains ( with a fermentation titer of 4800 - 5200u / ml by shaking flask ) such as a2 - 23 , a2 - 30 , asm6 and al - 16 were obtained
本文以尼拉霉素单一组分?安普霉素产生菌s . tenebrariusa04 (发酵单位为2847u ml )为出发菌株，通过对单孢子悬液和超声波破碎菌体的诱变处理并复合筛选模型，获得了遗传特性稳定的单组分高产菌株： a2 - 23 、 a2 - 30 、 asm6 、 a1 - 16 ，摇瓶发酵单位达4800 - 5200u ml 。 |
