Isolation and primary identification of a new nitrogng - fixation arthrobacter strain 一种有固氮能力的节杆菌菌株的分离和初步鉴定
2.
Multi - copies insertion transformants were screened on g418 plates . the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay . the n - carbamoylase activity of recombinant was 2 . 26 and 2 . 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169 将hyucdna片段连接到真核表达载体ppic3 . 5k上，经bgl酶切线性化，通过peg法转化导入毕赤酵母gs115感受态细胞，利用g418抗性筛选得到12个插入多拷贝目的基因的转化子。
3.
Puc18 - 169 was a subcloned plasmid containing the whole operon sequence of l - hydantoinase from arthrobacter bt801 which can convert 5 - benzylhydantoin to l - phenylalanine . hydantoin hydrolase which is responsible for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801 . n - carbamoylase is a part of hydantoinase operon which can transform n - carbamoylamino acids into the corresponding amino acids 节杆菌( arthrobacter ) bt801是由军事医学科学院生物工程研究所保存的l -乙内酰脲酶产酶菌株， puc18 - 169是由本室构建的含有节杆菌bt801的l -乙内酰脲酶完整操纵子序列的亚克隆质粒。
4.
The l - n - carbamoylase of arthrobacter bt801 , coded by the hyuc gene , is the rate - limiting and the only stereoselective enzyme . hydantoin hydrolase gene ( hyuh ) and n - carbamoylase gene ( hyu c ) were amplified from the plasmid of puc18 - 169 by pcr and , at the same time , activity expression was obtained in e . coli and pichia pastor is gs115 respectively 节杆菌bt801的乙内酰脲水解酶催化乙内酰脲类化合物水解开环， n -氨甲酰基氨基酸水解酶是该菌乙内酰脲酶系中惟一具有立体选择性的酶，也是整个反应体系的限速酶。
