According to the frequently used methods of extraction and isolation of natural products from organic body , the freeze - dried ascidian tunica was exhaustively extracted with 100 % chloroform then the combined extracts were filtered and the solvent was separately concentrated under vacuum to yield a dark - brown gum 按照目前比较常用的天然产物的提取分离办法，将样品冷冻干燥后，用100氯仿浸泡，收集氯仿滤液，减压蒸馏浓缩去除浸提溶剂，得到黑褐色的浸膏状物质。
2.
Considering of the specificity of the degenerative primer designed in this pcr reaction , the identity between the sequence we wanted and the fragment of pcr product and the presence of asmaspa , asmaspb , clr and cls ( the homologous gene of masp gene ) in halocynthia roretzi , a japanese ascidian , we believe that the sequence of pcr product is some part of the masp gene or masp homologous gene 基于本实验中所设计的引物为特异性简并引物，测序基因通过比较得到与预期片段有一定的同源性以及masp同源物asmaspa 、 asmaspb 、 clr和cls在海鞘中存在的事实，我们可以初步推测，本实验pcr反应所克隆的片段可能为文昌鱼masp或其同源基因的一部分序列。
