English translation for "bacmid"

杆粒[带有杆状病毒基因组的质粒，可在细菌和昆虫细胞之间穿梭

Example Sentences:

	1.	Transfection of the gp64 - mi and reintroducing hal33 gene bacmid dna into sf9 cells shown that hal33 can rescue the gp64 null bacmid in sf9 cells 对该重组病毒bv的westernblot检测，结果表明fi和fz同时存在并以二硫键相连。
	2.	The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme , were linked by means of t4 dna ligase and transformed into e . coli jm109 permissive cells , yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid 将重组质粒pugedna与转移载体pfastbacldna用bamhi和ecori双酶切处理， t _ 4dna连接酶连接，用连接产物转化大肠杆菌jm109感受态细胞，得到重组转移载体质粒pfastbac - gedna 。
	3.	The interest gene was inserted in the - tha l . tho 1 multiple cloning sites of donor plasimd pfastbachtb of baculovirus expression system . after analysis by restriction endonuclease and pcr , the recombinant donor plasmid gpl - fast was transforn1ed to the competent celi dhi0bac which contalns the bacmid and the helper plasmid , the recombinant bacmid gpl - bac was acquired which would express the vpl of gpv strain h l 同时将该目的基因插入到杆状病毒表达系统的供体质粒pf _ ( ast ) b _ ( ac ) htb的xba 、 xho多克隆位点间，经酶切、 pcr鉴定后，将重组的供体质粒gp1 - fast转化到含有杆状病毒和辅助质粒的dh10b _ ( ac )感受态细胞中，获得了表达gpvh1株vp1的重组杆状病毒gp1 - bac 。
	4.	I ) two mutants ( hpab - 38 and hpab - 34 ) were designed based on the three - dimension structure of hpab - constructed by protein homology modeling method , ii ) the mutant molecules were generated by pcr and inserted into pfast hta donor plasmid , the later was then transformed into escherichia coli dhlobac to generate recombinant baculoviruses dna by site - specific transposition of an expression cassette into a baculovirus shuttle vector ( bacmid ) propagated in e . coli . the recombinant bacmids were isolated and transfected into insect sf - 9 cells to reproduce baculoviruses 4 . h队b一p及其突变体高效表达工程菌的构建:将用pcr扩增的h队b一p 、 h狱b一p35 、 h队b一p34基因，分别与pinpointxa一3质粒连接，转化jm109大肠埃希菌，获得的工程菌在表达融合蛋白时不稳定，第5代后即见不到表达条带。

Similar Words：

"bacllus anthracis" English translation, "bacllus butyricus" English translation, "baclofen" English translation, "bacmecillinam" English translation, "bacmeister" English translation, "bacnet" English translation, "bacnotan" English translation, "baco" English translation, "baco mt" English translation, "baco tone" English translation