Callose stains are aniline blue, resorcin blue and rosolic acid . 胼胝质的染剂是苯胺蓝，间苯二酚蓝和玫红酸。
2.
( 4 ) fluorescent investigation shows that pollen started to germinate about 20 hours later after deposited on the stigmas , only 1 or 2 pollen tubes can penetrate ovary to micropylar end on the fifth day . callose plugs evenly formed in the pollen tube , and sometimes callus deposited randomly along pollen tube wall and even the whole tube wall . ( 5 ) 16 species of insects visited c . praecox in nanjing , belonging to hymenoptera , diptera , coleoptera , thysanoptera and spiders ( arachnoidea ) ; apis cerana cerana fabricius , aldrichina grahami ( aldrich ) , epistrophe balteata de gee , musca hervei villeneuve are its main efficient pollinators ( 5 )南京地区蜡梅的访问昆虫约16种，分属膜翅目hymenoptera 、双翅目diptera 、鞘翅目coleoptera ，缨翅目thysanoptera 、及蛛形纲arachnoidea ，主要传粉昆虫有4种：膜翅目蜜蜂科东方蜜蜂中华亚种apisceranaceranafabricius 、双翅目蝇科muscidae黑边家蝇muscaherveivillcneuve 、丽蝇科calliphoridae巨尾阿丽蝇aldrichinagrahami ( aldrich )及食蚜蝇科syrphidae黑带食蚜蝇epistrophebalteatadegeer ；亮叶蜡梅的访问昆虫约18种，分属鞘翅目coleoptera 、半翅目hemiptera 、缨翅目thysanoptera 、膜翅目hymenoptera 、双翅目diptera 、鳞翅目lepidoptera ，另外有蛛形纲arachnoidea的蜡橄ch俪ononthuspr口ecox ( l
3.
The results were as follow : in the process of its male organ development , these are many abnormalities , such as : premature tapetal degradation , giant tapetal cells , the great vocalization of the tapetal cells and inadaptable function of callose enzyme , which lead abnormal and premature degradation microspores and male gametophytes 实验结果表明： 1猬实的雄性生殖器官在发育过程中，出现绒毡层细胞的液泡化、肥大、提前降解及不适时释放胼胝质酶等异常现象，造成猬实花粉发育过程中出现畸形、解体。
