Effect of total coumarins of fructus cnidii on osteoblast in neonatal rat calvaria culture 蛇床子总香豆素对新生大鼠成骨细胞的作用
2.
The insulin - like growth factor - 2 ( igf - 2 ) and igf conjugated protein - 6 ( igfbp - 6 ) mrna level in rat calvaria bone tissue and mc - 3t3 - el cells were detected by northern blotting analyses and reverse transcription polymerase chain reaction . the estrogen responsive element ( ere ) in igfbp - 6 gene promoter was identified and involved in tcdd - reduced regulation of the gene expression by electromobility shift assays ( emsa ) 正常胎鼠头盖骨组织igf一2mrna呈高水平表达状态，而igfbp一6mrna的水平较低; ccf胎鼠头盖骨骨组织内igf一2mrna的表达较正常胎鼠降低， igfbp一6mrna的表达则明显升高; atra和e :联合应用时， atra可以抑制雌激素对细胞内igf一2和igfbp一6的这种调节作用。
3.
The protein nucleic acid complexes were identified by autoradiography for 48 h . the psg5heo and igfbp - 6 promoter pcat vectors were transfected into cos - 7 cells with iipofectamin2000 ; these cos - 7 cells where treated with 1 jxm e2 , with lonm tcdd and with both lonm tcdd and 1 jxm e2 , individually ; the cat protein was examined by cat - els a . results : the congenital skeletal defects in fetal rats were induced with 10 - 15ug / kg tcdd or with 100 - 140 mg / kg ra with dosage - dependence effect . the igfbp - 6 mrna in rat calvaria tissue and mc - 3t3 - el cells were increased with tcdd and ( or ) ra , whereas the cell proliferation and igf - 2 mrna in mc - 3t3 - el cells was decreased in the status of e2 结果: tcdd在大鼠胚胎发育时期具有骨骼致畸作用，骨骼发育畸形包括:内翻足、脊柱裂、颅面骨发育缺陷、无尾畸形等;随着tcdd浓度的升高( 10一15林扩kg ) ，致畸率明显上升( 14 . 7 %一24 . 6 % ) ; tcdd和ra联合用药可以增强对大鼠骨骼发育的致畸效应; e :对成骨细胞内igf一2基因有正性调节作用，对igfbp一6基因有负性调节作用， tcdd可以抑制e :对细胞内xgf一2和igfbp一6的调节作用。
