It was established that grb - ast3 concatemer are more easily expressed in the way of fusion expession 初步认为grb - ast _ 3串联体基因适宜采用融合表达载体表达。
2.
Is encoded by grb ~ ast7 . the expression analysis of the concatemer was proceeded in the e . coli pet expression system 本文主要工作就是构建grb - ast _ 7串联体，将构建后的串联体导入大肠杆菌进行表达分析。
3.
Neuropeptide ast2 is encoded by grb - ast3 . pcr methods were tried to construct grb - ast3 concatemer . the expression analysis of the concatemer was proceeded in the e . coli pet expression system 本论文采用改进的pcr “自身模板-引物”法进行构建方法构建grb - ast _ 3串联体，并将拼接好的串联体转入大肠杆菌进行表达分析。
4.
Concatemer were constructed by polymerization with t4 dna polymerase and primer - templated pcr with high fidelity dna polymerase . one concatemer of 360bp , 9 copies of grb - ast3 , were obtained from the first pair of primer and three ones of 515bp , 750bp , 792bp , from the second one 从第一对引物的拼接产物中克隆到一个360bp含有9个拷贝数的grb - ast _ 3的串联体，从第二对引物的拼接产物中克隆到三个分别为515 、 750 、 792bp的grb - ast _ 3的串联体。
5.
In this method , high fidelity dna polymerase ( pyrobesf * dna polymerase ) was used to ensure efficient and accurate amplification . after cloning and sequencing of the pcr products , one concatemer contains seventeen copies of grb ~ ast7 was attained . the grb - ast ? concatemer was cloned into pet22b expression vector 先用t4dnapolymerase构建成模板，再通过高保真dna聚合酶( pyrobesttmdnapolymerase ) pcr进行扩增，从中得到一个含有17个拷贝的grb - ast _ 7的串联体。
