In this paper , we constructed the genomic dna library of nephila clavipes with the vector supercos 1 cosmid 以dig - oligo2为探针，菌落原位杂交筛选cosmid文库，得到56个阳性重组子。
2.
The me2155 genomic library was constructed using phz132 as the cosmid vector , and 10 positive clones were fished out using 0 . 9kb pcr product as probe 以phz132为载体，构建了mc ~ 2155的基因组文库。以0 . 9kb的扩增产物为探针从文库中钓出10个阳性克隆子。
3.
Strain sa - coo by southern hybridization . a cosmid - based gene library of streptomyces griseus atcc14811 was constructed using phz1357 , a streptomyces - e . coli bifunctional vector carrying two cohesive sites 为了获得胆固醇氧化酶基因，以大肠杆菌-链霉菌双功能柯斯质粒phz1357为载体，构建了灰色链霉菌atcc14811的基因组文库。
4.
The two end - points were localized on a 4 . 3kb bamhl - ecor1 fragment of a cosmid 16c3 and a 1 . 2kb sail fragment of a cosmid 17g7 respectively . the deletion junction was localized on a 2 . 8kb bamhi fragment of the zx1 chromosome 两个端点分别定位在野生型菌株基因组文库粘粒16c3的4 . 3kb的bamh1 - ecor1片段上和17g7的1 . 2kb的sal片段上，缺失界点定位在zx1染色体上的一个2 . 8kbbamh片段上。
5.
Then , we find out the write clones through the chemis hib . during the working , three probes which we used are from the published sequences of spidroin . after this , a genie library of subclone of dragline was constructed 然后我们从构建蛛丝蛋白的亚克隆文库着手，采用鸟枪法测序的策略，取从蜘蛛cosmid文库中经过证实的阳性克隆scos - ds1 ，大，小为40kb左右，用cai ， eco47 ，和hinf三种酶分别对其进行酶解分析。
6.
A gene library of psedomonas fluorescens g2 was constructed in the cosmid vector pla2917 using e . coli jm109 as the host strain . two recombinants , pgr3 and pgr7 , which can confer glyphosate resistance ofe . coli jm109 were identified from the selective medium containing 10mm glyphosate 以粘粒pla2917为载体、大肠杆菌jm109为受体菌构建荧光假单胞菌g2的基因组文库，在含有10mm草甘膦的固体选择培养基上筛选出两个耐受克隆pgr3和pgr7 ，插入片段分别为7kb和11kb 。
