English translation for "cotransfection"

共转染

Example Sentences:

	1.	Interaction with doc - 1r and cdk2 proteins under physiological condition using cotransfection , coimmunoprecipitation and western blotting , we have transfected the plasmids into 293 cells according to following combination : pc 分别将正、反义小鼠doc一ir重组体命名为pedna3一doc一ir +和pedna3一doc一ir一。
	2.	The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase , to make the scfv - linker - uk32 chimeric gene . this gene was cloned into the transfer vector pbacpak9 , and cotransfected with bacpak6 bsu36i digest into sf 9 cells . the fusion protein was secreted into the medium . in the fifth day after the cotransfection , the supernatant of the medium showed 107 iu ml fibrinolytic activity , higher than 25 iu ml fibrinolytic activity of scfv - uk32 . elisa showed that the supernatant had the binding activity to activated platelet . wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain 为了提高重组导向溶栓分子scfv - uk32的溶纤活性，通过重组pcr方法在编码scfv与uk32的碱基之间引入编码klgggg连接肽的碱基序列，并克隆到转移载体pbacpak9上,通过与线性病毒dna bacpak6 bsu36i digest共转染到昆虫细胞sf 9内，进行表达。表达产物分泌到上清中，共转染后第5d天用纤维平板法测得sf 9细胞上清溶纤活性达到107 iu ml ，比未引入连接肽的scfv - uk32的表达活性25 iu ml高。

Similar Words：

"cotralha" English translation, "cotran" English translation, "cotransaminase" English translation, "cotranscript" English translation, "cotransduction" English translation, "cotransformation" English translation, "cotranslational" English translation, "cotranslational cleavage" English translation, "cotranslational glycosylation" English translation, "cotranslational integration" English translation