| 1. | The cyanophage contained a double - stranded dna genome at ca . 36 . 6kb 病毒的遗传物质为双链dna ,基因组的大小约36 . 6kb 。 |
| 2. | More studies were conducted on both the physiology and ecology of the cyanophage 在上述研究的基础上,我们还系统开展了噬藻体生理与生态特性的研究。 |
| 3. | Investigation in details on a freshwater cyanophage isolated in china infecting plectonema and phormidium is presented in this thesis 我们在国内首次分离和报道了淡水噬藻体(蓝藻病毒) ,其宿主为织线藻和席藻。 |
| 4. | Results involved in measuring burst size and latent period of the cyanophage showed that both temperature and sunlight are the important effect factors 并且,发现当温度和光照分别为28和2000lux时,吸附率达到最大值为62 . 4 。 |
| 5. | Under trasmission electronic microscope ( tem ) , the cyanophage showed a bacteria - phage shape with head diameter at 52nm and short tail ( almost invisible ) 电镜照片显示该噬藻体为噬菌体形态,头部直径为52nm ,尾部很短,几乎不可见。 |
| 6. | Under the dark condition or at low temperatures , the cyanophage would n ' t multiply , but the elevation in both temperature and sunlight would bring about the burst size to increase 测定释放量和潜伏期的实验结果表明,温度和光照是主要的影响因子,在无光照或温度较低的情况下,噬藻体几乎不增殖。 |
| 7. | Furthermore , the sequences were analyzed and compared with the sequence data in genbank . the results indicated that the cyanophage represented a phage - like shape with head diameter ca . 47 - 52nm and a short tail almost invisible 研究结果表明:此病毒在负染电镜下为噬菌体形态,头部直径约47 - 52nm ,尾部几乎不可见。 |
| 8. | The burst size of the cyanophage was ca . 200 pfu ? cell " 1 . the rate of adsorption to host was markedly higher under light condition than that under dark condition ( p = 0 . 02 ) , and light was necessary for infection 此外,固体培养条件下,织线藻的生长速率与噬藻斑的扩大之间有明显的同步性,即生长速度越大,噬藻斑的扩大就越明显。 |
| 9. | This method was also effective for concentrating virus form large volume water samples . moreover / the concertration multiple of cyanophage pp could rise to nearly one - hundred times when two - step backflushing ultrafiltration technique was adopted 此法也适用于大量水样的处理,而且当采用两步反冲超滤技术时,噬藻体的浓缩倍数可以提升到近100倍。 |
| 10. | Several methods for measuring the lysing cycle and burst size of the cyanophage were tested . it was established that cell counting method was more convenient and exact for confirming the lysing cycle . burst size obtained by 1 pfu infecting was reliable 我们研究了确定该噬藻体裂解周期和释放量的多种方法,认为细胞计数法是测量裂解周期既方便又准确的方法,而采用1pfu的感染可获得噬藻体的准确释放量。 |