| 1. | The generation of endostatin is catalyzed by proteolytic enzymes , that cleave peptide bonds within the protease - sensitive hinge region of the c - terminal domain
特异性蛋白水解酶降解胶原c末端的蛋白酶敏感区，产生内皮抑素。 |
| 2. | Sequencing and genbank blasting demonstrated that these four had the same sequences and encoded part of arabidopsis choloroplast carbonic anhydrase ( ca ) . the coding region contained 251 amino acid , from n terminal amino acid 86 to c terminus
到genbank进行序列比对，结果表明它们编码拟南芥叶绿体碳酸酐酶( ca )基因，编码区含有部分ca序列，从n端第86位氨基酸至c末端，共251个氨基酸。 |
| 3. | We obtained two positive clones after screening . sequencing and blasting results showed that the two clones had the same sequences and encoded part of ked like protein in arabidopsis . the coding region was from n terminal amino acid 240 to c terminus
测序及序列比对结果表明二者序列相同，为ked样蛋白的部分编码序列，编码部分从n端第240位氨宋林夜:利用酵母双杂交系统筛选与g蛋白及cam相互作用的新型蛋白基酸至c末端，共207个氨基酸。 |
| 4. | To further enhance protein productivity of expression vectors , two artificial transcription activating domains , ah and vp2 , were linked to cl represser protein of phage through a soft linker , respectively , and thus two artificial transcription activators were created
为了进一步提高表达载体表达外源蛋白的能力，我们把两个人工转录激活结构域ah和vp2分别通过一个柔性的linker融合到噬菌体ci蛋白的c末端，构建了两个人工转录激活因子。 |
| 5. | 3 . preparation of polyclonal antiserum against angiotensinogen in order to study agt in protein level and also for other continuing work , the c - teminus of rat angiotensinogen was expressed in e . coll . rabbits were immunized by this expressed 6his - agtc protein and serum from different rabbits were raised
血管紧张素原多克隆抗血清的制备为研究agt蛋白表达及后续工作，在大肠秆菌中融合表达了agt蛋白的c末端，以此融合蛋白为抗原免疫家兔，制备抗血清。 |
| 6. | Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115 . mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究将含有n末端信号肽和c末端毒性区缺失的pap基因的表达载体ppic9k - p用sali酶切线性化后，通过电击转化整合p . pastorisgs115菌株细胞中。 |
| 7. | A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein . the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis . it was demonstrated that constructed phagemid can still express penicillin g acylase
将包含信号肽和琥珀终止密码子uag ( amber )的完整巨大芽孢杆菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ，通过引入的11肽连接青霉素g酰化酶的c末端与噬菌体外壳蛋白gp3的n末端。 |
| 8. | A change in functional properties of the nmda receptors has been invoked as a potential mechanism contributing to the loss of synaptic plasticity during brain maturation . from this , it is important to well understand the structural and functional diversity of nmda receptors in relation to nmda receptor subunit composition and spatial distribution at excitatory synaptic sites during development
近几年来，在哺乳动物中枢神经系统中，对离子型谷氨酸受体如何定位到兴浙江大学医学院硕士论文nrzb亚单位的c末端胞内区影响n协da受体装配、运钧以及表面表达的分子机制奋性突触的研究有了长足的发展。 |
| 9. | The mouse fertilized egg is the most simple and natural mammalian model of cell cycle , there are few reports about the mechanism on the regulation of early development of mouse fertilized eggs especially the events of entry into m - phase . its the first time we report here that pkb exists and effects on g2 / m phase transition in mouse one - cell stage fertilized eggs
148 - 411是激酶区(催化域) ，和其它丝苏氨酸蛋白激酶有较高的同源性，其中有一个保守的thr ，它位于308 、 309或305位；从412位起为c末端尾区(调节域) ，除pkb -外都有一个保守的ser ，位于473或474位。 |
| 10. | Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e . coli bl21 ( de3 ) . no squalene synthase polypeptide of expected molecular mass was observed in e . coli containing the putative full - length squalene synthase cdna , however , overexpression in e . coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus
但在含有全长的鲨烯合酶cdna的大肠杆菌中并没有观察到预期大小的鲨烯合酶表达，而c末端截短30个疏水氨基酸的鲨烯合酶可在大肠杆菌中过量表达。 |
