| 1. | Calmodulin - dependent cyclic nucleotide phophodiesterase was prepared from bovine brain by two - step deae - cellulose column chromatography
摘要通过两次离子交换柱层析，从牛脑中制备钙调素依赖性的环核苷酸磷酸二酯酶。 |
| 2. | 3 . successful purification of the crude ha was achieved by anion exchange resin deae - cellulose column chromatography using 0 . 6mol / lnacl solution as an eluant
3 .采用阴离子树脂deae一纤维素对透明质酸粗品进行纯化，当naci为0 . 6mol / l时，蛋白质含量较低。 |
| 3. | An active metabolite was obtained by purification with precipitated by ethanol , sephadex g - 25 gel , deae - cellulose ion exchange resin and silica gel column chromatography
经乙醇( 95 )沉淀、 sephadexg - 25凝胶层析、 deae -纤维素离子交换层析和硅胶层析纯化，得到抑制黑曲霉生长的单一组分。 |
| 4. | But the whole level of serum titer in combined vaccine group was higher than others . igg was extracted by salting out with ammonium sulfate and purified by ion exchange chromatography with deae cellulose
对分离到的血清用饱和硫酸铵盐析法提取igg ，并用deae纤维素离子交换层析法对提取的igg进行纯化。 |
| 5. | The purification of this halocin was achieved by combination of tangential flow filtration ( tff ) , sephadex g50 and deae - sepharose chromatography . the n - terminal amino acid sequence was also determined by edman degradation
Halc8对大多数的极端嗜盐古菌(包括部分嗜盐碱古菌)有抑制作用，但对细菌则无明显的抑制作用。 |
| 6. | Methods 1 mr1 hybridoma was cultured in hollow fibre system . culture supernatant was harvested , then precipitated by half - saturated ammonia sulphate . mfm was purified by deae - sephacel ion exchange chromatography . mri concentration was measured by elisa
硫酸钱半饱和沉淀， oeae阴离子交换层析分离纯化抗co40l单克隆抗体mri 。 pbs透析后， 505一队ge检测纯化抗体的纯度， eusa测定抗体浓度。 |
| 7. | In this optimal condition , p450nor was expressed massively . the expressed product was then purified by deae - cellulose chromatography . the purified expressed protein showed one band in sds - page and the purification attained anticipative purpose
在此条件下，大规模诱导表达重组细胞色素p450nor ，经deae纤维素色谱柱纯化， sds - page分析表明，纯化的目的蛋白基本上为单一谱带，纯化达到预期效果。 |
| 8. | In this paper , by shorting the length of deae - cellulose column and increasing the ph of elution solution , one kind of go isozyme was purified rapidly from green leaves of spanictu brassica parachinensis bailey and vigna unguiculatew . ssp . sesquipedalis ( l )
本文通过一种改进后的方法，即通过缩短deae - cellulose - 52柱的长度，以及提高洗脱液的ph ，可在9h内从菠菜、菜心和豆角绿叶中纯化go同工酶。 |
| 9. | The crude cellulases from liquid fermentation of b - 6 and ass . 3711 were isolated and purified by ( nh4 ) so4 precipitation , sephadex g - 100 and deae - sepharose cl 6b column chromatography . the cmcase components were purified and some of their physical and chemical properties were studied
本文将液体发酵的酶液经硫酸铵分级沉淀、柱层析后得电泳纯cmcase组分，并对as3 . 3711和b - 6来源的cmcase酶解动力学和理化性质作了比较研究。 |
| 10. | Without ammonium sulphate fractionation and dialysis , the supernatant of crude extract was directly loaded on deae - sepharose cl 6b column equilibrated by phosphate buffer ( 50mmol / l , ph7 . 8 ) , and the enzyme fraction was not absorbed on the column but impurities were absorbed
粗酶液无需硫酸铵沉淀及透析，即可引入磷酸缓冲液( 50mmol l ， ph7 . 8 )预平衡的deae - sepharosecl6b柱，上柱后用平衡缓冲液洗至基线稳定。 afpga不被吸附而直接流出。 |
