The study of denaturalization of soy protein during the extraction of soy isoflavone 大豆异黄酮提取过程中大豆蛋白变性的研究
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The main equipment of the ion electrostatic accelerator is in good condition . if it is used again it will be the important implement of science research about atomic nucleus physics , biology aberrance and material denaturalization 它的主要设备情况良好，一旦恢复使用，将成为原子核物理，生物诱变，材料改性等科研工作的重要工具。
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Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation . 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies . 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs , prepare a 50 % slurry for batch purification method , pack column with matrix slurry 三、 gst一hbrp重组蛋白的纯化1 .超声破碎细胞，离心，上清和沉淀进行sds一page电泳分析2 .样品处理提取包涵体，变性后，加人用pbs平衡过的以utal肠onesepb抓脱4b ，室温下孵育3 .以utadtionesepharose4b柱纯化以utad云onesepharose4b柱的准备根据毛山lel ，决定纯化所需的gll卫ta1如oneseph娜se4b的柱床体积，用预冷的1xpbs清洗cldtathionese户， se4b ，得到50 %的基质
