| 1. | An about 550 bps and an about 700 bps fragment could be seen after the vector pqe80l / dhfr / hpd - 3 was digested by bamh i + kpn i and by bamh i + hindiii r
经w亡sternblot证实，在大小分别约为3ikd和26kd的位置有两条清楚的蛋白带。 |
| 2. | The recombinant vector pqe - 80l / dhfr / hpd - 3 was identified by restriction digestion while we also construct the recombinant control vector pqe80l / dhfr with the same strategy . 3
同时还将表达运载蛋白dhfr的基因单独连接于表达载体pqe - 80l ，得到对照质粒pqe - 80l dhfr 。 |
| 3. | ( 2 ) the construction of the recombinant prorarylic expression vector pqe80l / dhfr / hpd - 3 and the control vector pqe80l / dhfr . an about 135 bps fragment could be seen after pqe80l / dhfr / hpd - 3 vector was digested by kpn i + hindiii
Sds一队ge分析可见重组原核表达载体pqe一80l / dhf侧hpd一3表达的目的蛋白带和对照质粒pqe一sol / dhfr表达的蛋白带，大小分别约为31kd和26kd 。 |
| 4. | 2 . to construct the prokaryotic expression vector and the control vector . after sequenced , the target dna fragment was cloned into pqe - 80l vector together with the dna fragment encoding carrier protein dhfr
将自puc18 h d - 3回收的目的片段连同表达运载蛋白dhfr的基因片断一起连接于原核高效表达载体pqe - 80l ，经酶切鉴定，得到重组原核表达载体pqe - 80l dhfr h d - 3 。 |
| 5. | The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue , then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells . after screeened with g418 and cloned , 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons . it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
抽提、鉴定、纯化重组质粒后，脂质体转染法将重组质粒导入中国仓鼠卵巢细胞( cho - dhfr ~ - )中， g418选择转染子并克隆化培养，经rt - pcr和分子灯塔探针杂交鉴定其mrna转录，获得4株稳定表达54位密码突变型mbl的cho细胞。 |
| 6. | Three kinds of integron were detected from 7 s . typhimurium with a size of 638bp , 1913bp and 2013bp respectively . the products were cloned and sequenced . the results showed that they contained two aada2 gene , one dhfrxii gene associated with the resistance
序列分析表明，这7株鼠伤寒沙门氏菌的型整合子可分为三种类型，一种大小为638bp ，存在1个阅读框；另一种大小为1913bp ，含1个aada2 、 1个dhfr基因盒和1个阅读框；第三种大小为2013bp ，有1个aada2基因盒和1个阅读框。 |
