| 1. | The physical and chemical characters and secondary structure of apoptin were predicted with softwares antheprot and dnastar
本实验利用antheprot和dnastar软件对凋亡素的理化性质和高级结构进行了预测，并在genbank中进行了blast分析。 |
| 2. | On the other hand , dnastar software was utilized in the analysis of the different segments of bdnf genes . the reasonable phylogenic tree was constructed with the leading peptide encoding sequences
运用软件dnastar中的megalign程序分别对28种动物bdnf基因的不同区段进行分析，发现采用前导肽区编码序列能够得到比较正确的系统树。 |
| 3. | The homology of nucleotide sequence between b , w strain and domestic isolated strains were 98 . 4 % ~ 99 . 5 % and the homology of amino acids were 97 . 3 % ~ 99 . 1 % . phylogenetic tree analysis showed there was a high relationship between gxb , w strains and ea strain
为了进一步了解广西两毒株与国内外毒株之间的亲缘关系，用dnastar软件做了遗传树分析，结果表明b 、 w株与ea株的亲缘关系最接近。 |
| 4. | Total rna was extracted from the second stage larve of hypoderma sp , then single chain cdna was synthesized by reverse transcription using oligo ( dt ) 18 as a primer . the hypodermin c ( hc ) and hypodermin a ( ha ) gene specific primers were devised by dnastar software
本试验的目的旨在进行hypodeminc ( hc )和hypodermina ( ha )基因的克隆、测序、构建重组表达载体并诱导表达，获得重组抗原，以解决天然抗原的不足并为诊断和免疫试剂的产业化奠定基础。 |
| 5. | The obtained haplotypes are compared , aligned and analyzed by dna sequence analysis software package dnastar , dnasp , mega , to calculated residue composition , polymorphic sites and genetic distance , to consider the types and rates of residus substitution and to create neighbor - joining ( nj ) phylogenetic trees and determine phylogeny of the classification taxa involved in the research . 5
5 、盲榕科昆虫cytb基因序列分析方法和分析结果直接反映着被试分类单元的遗传物质的差异和遗传本质，是最为客观的系统分类依据，所以可应用于盲峙科的种类的鉴别、种群的分析、种上类群的划分以及科级阶元水平系统发育关系的确定。 |
| 6. | The purpose of this study was to elucidate the biological function of hnadcs and its relationship to renal disorders . methods : the biochemophysical characteristics and secondary structure of amino acid sequence of hnadcs were analysed via software on - line ( http / / expasy . pku . edu . cn / cgi - bin / ? protparam ) , and the antigen epitope was predicated via software dnastar
郑州大学2002年硕士毕业论文人钠h被匹协同转运目白zk合蛋白抗体的制击皿其在瞩脏的这达研究方法：首先利用互联网上软件mp ： llexpasypku ? edu ? cn7cgl七inlprotparam ，分析蛋白理化性质和二级结构，应用生物技术软件dnastar预测hnadc3抗原表位。 |
| 7. | The complete nucleotide sequences of two strain viruses were determined . ten pairs of primers were designed based on the nucleotide sequences of tbev sofjin - ho , oshima5 - 10 by dnastar software . the virus cdna was expanded by rt - pcr in ten parts . sequenced by clones method . 5 " terminal and 3 " terminal cdna was expanded by race method . the nucleotide sequences and deduced amino acid sequences of two strain viruses were compared with the sequences in genbank by dnastar software
根据genbank中已公布的tbevsofjin - ho株和oshima5 - 10株核苷酸序列利用dnastar软件设计10对引物，利用rt - pcr技术分段扩增病毒cdna ，克隆法测序。病毒基因组5末端和3末端序列采用race法扩增。测序结果用dnastar软件拼接，在genbank中检索6株tbev编码区核苷酸序列、 12株tbeve蛋白核苷酸序列，并在dnastar上进行核苷酸序列、由此推导的氨基酸序列比较及构建系统发生树。 |
| 8. | At first , 1 . 67 u g per well mcab all was coated on three wells of a plate , and then 1 . 5 x 1011 phage virion was diluted and added , after incubating with the target , wash away unbound phage by tbst ( 0 . 1 % tween - 20 ) , the bound phage was eluted with ph 2 . 2 tris - gly buffer and amplified , the specially bound phage was enriched by taking through addition binding / amplification cycles . ln the following cycles , the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all , collecting and titering the washing phage of last time and output phage in each round , the selective ratio and the false positive rate of each round were worked out , the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective . after 4 rounds of panning , 11 phage clones were selected after competitive - ellsa , the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced , a clear consensus binding sequence emerged
在本实验中，利用随机12肽库对抗猪瘟病毒( classicalswinefeverviruscsfv )糖蛋白me2的单抗a11进行表位筛选，经过四轮筛选以后，随机挑取11个克隆作竞争- elisa检测，结果表明，所挑11个克隆中，有9个克隆能对me2蛋白和a11反应产生抑制作用，抑制率最高可达64 ； dna测序以后经过dnastar软件分析，发现它们的核心序列为anwralsl ，该核心序列与猪瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夹心- elisa检测和western - blotting试验均证明所挑阳性克隆能被a11所识别；人工合成含核心序列的多肽经间接elisa试验证实，也能被a11识别。 |
| 9. | The n - terminal nucleotides 47 - 420 of the guangxi apmv - 1 isolates of different poultry species origin were amplified and sequenced . the alignment and phylogenetic analysis of the nucleotide sequences and deduced amino acid sequences of f gene of the guangxi isolates and other reference strains obtained from genbank were done
研究对鸡、鹅、鸽三种禽源apmv刁广西分离株f基因n与前段进行了rtpcr扩增及核茸酸序列测定，并用基因分析软件dnastar进行分析并与已发表的其它参考毒株进行比较。 |
| 10. | 374 - nt sequence analysis between nt 47 - 420 and restriction enzyme ( re ) clevage site mapping of f gene between nt 34 - 1682 were used to compare the 18 isolates for genetic analysis . a phylogenic tree was constructed based on the 374 - nt - sequence data of eighteen isolates in the study and 37 ndv reference strains from genbank and published resources
通过dnastar软件对f基因47 470nt间片段进行同源性分析比较并绘制了遗传进化树枝状结构发生图，结合334 1672nt间三种限制性内切酶( re ： hinf ， bsto及rsa )位点的分布情况，确定了这些分离株的基因分类地位。 |
