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English translation for "dsrna"

rna]双链rna
双链rna


Related Translations:
dsrna病毒:  rna virus
isometric dsrna mycovirus:  等长双链rna真菌病毒等长双链rna真菌簿
Example Sentences:
1.Group iii : dsrna viruses
双链rna病毒
2.Rnai ( rna interference ) is a process by which double - stranded rna ( dsrna ) specifically suppresses the expression of a target mrna . since fire et . al
Rnai ( rnainterference )是双链rna介导的、序列特异的同源基因转录后的沉默效应。
3.According to the reported complete vp6 nucleotide sequences of group a prv in genbank , a pair of primers was designed to amplify vp6 gene of jl94 with oligo 4 . 1 software . the segments of complete gene 6 of jl94 were obtained from rt - pcr using dsrna extracted from the virus as template
根据genbank中的多株猪a群轮状病毒vp6蛋白完整基因序列,利用oligo4 . 1设计引物,以jl94rna为模板,通过rt - pcr扩增出长约1 . 3kb的基因片段,命名为vp6 。
4.These results showed that the three isolates were infectious bursal disease virus . the full - length cdna of the genomic segment a of two viruses , one virulent field strain ibdv zj2000 and one attenuated strains ibdv jd1 , were amplified in a single step procedure by long - accurate reverse - transcription polymerase chain reaction ( la - pcr ) , cloned into pgem - t easy vector , and sequenced
分别以传染性法氏囊病病毒zj2000株(野毒株)和jd1株(弱毒株)基因组dsrna为模板,采用long - accuratert - pcr ( la - pcr )一步法扩增并克隆了两株病毒的基因组a节段全长cdna 。
5.According to the gene sequence and secondary structure of hcv ns5b , we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et . al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion , the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr
然后根据dsrna设计原则,结合nssb基因的序列特征,借助生物信息学软件设计了针对nssb基因的sirnas ,并交由公司化学合成;电穿孔法转染上述稳定转染的细胞克隆,同时分别以非特异的sirnas转染组和空白转染组为对照, dapi染色后通过荧光显微镜和内标化rtpcr检测,初步证实了化学合成的sirnas可以特异阻断nssb基因的表达。
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