| 1. | Equine infectious anemia virus ( eiav ) is a member of the lentivirus of retrovirusae and the causative agent of equine infectious anemia ( eia )
马传染性贫血病毒（ eiav ，简称马传贫）是反转录病毒科慢病毒属的成员之一，是马传染性贫血病（ eia ）的病原体。 |
| 2. | The chinese donkey leukocyte attenuated strain of eiav （ dla ） was the only successful lentivirus vaccine up to now , which has been applied extensively in china against eia
我国的eiav弱毒疫苗是迄今为止世界上唯一投入应用的慢病毒疫苗，成功地控制了我国eia的流行。 |
| 3. | Chinese equine infectious anemia virus donkey - leukocyte attenuated strain ( eiav - dla ) is the unique lentivirus vaccine which has been used in large range for more then 20 years in china mainland
该病以间歇热，贫血、消瘦为特征，呈慢性进行性，终生持续感染，以死亡而告终，少数病例可发生急性死亡。 |
| 4. | Equine infectious anemia virus ( eiav ) , a macrophage - tropic lentivirus , causes persistent infections of horses characterized by rapid development of acute disease and following a long chronic and persistent phase
马传染性贫血病毒( equineinfectiousanemiavirus ， eiav )是反转录病毒科慢病毒属的重要成员，是引起马属动物传染性贫血病的病原。 |
| 5. | The vaccine was developed by passaging eiav strain liaoning ( eiav l ) in donkeys in vivo , obtaining a donkey - adapted virulent equine infectious anemia virus ( eiav da ) and then passaging it in donkey leukocyte cultures for more than 120 times in vitro
该毒株是将eiavl株（ eiavl ）通过驴体传代，使其毒力明显增强，然后在驴白细胞培养物上连续传代致弱获得的。 |
| 6. | Because of difficulty in differentiating eiav wild strain from the vaccine strain （ dla ） in vivo ， we have to build a fast identification method between wild strain and vaccine strain of eiav s2 is an accessory protein of equine infectious anemia virus ( eiav ) , the s2 function is not fully defined
由于我国马传贫弱毒疫苗存在野毒和疫苗毒鉴别困难的问题，目前尚未得到欧美等发达国家的认证，如要进入国际市场，急需建立一种eiav强、弱毒快速鉴别诊断方法。 |
| 7. | An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv . the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined
将包含全基因片段的三个基因克隆以限制性内切酶消化后顺次连接克隆到载体ptz18r上，构建了4个全长基因的分子克隆，分别命名为pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中两个转移到低拷贝载体plg338上，命名为plgd30343 、 plgd70344 。 |
| 8. | The eiav - pok8 . 2 - his was transfected into the donkey leukocyte culture . following an incubation period , reverse transcriptase activity was detected in cell culture supernatants . cytopathic effect was observed by no . six passages post - infection in donkey leukocyte infected by the virus derived from eiav molecular clones eiav - pok8 . 2 - his
提取前病毒dna ， pcr扩增p1p4片段，亚克隆至pmd18 - t载体，测序证明前病毒dna含有六个组氨酸，从而在体外获得了感染性克隆病毒株。 |
| 9. | It has been reported that the eiav s2 is not essential and does not appear to affect virus infection and replication in vitro . thus , we introduced a his - tag into the s2 gene of an eiav infectious molecular clone recombinant plasmid ( pok8266 ) by using soe pcr method and obtained a new recombinant plasmid with his - tag , designated as eiav - pok8 . 2 - his
本研究应用已构建好的eiav驴白细胞弱毒疫苗株的感染性分子克隆载体（ pok8266 ）为模板，通过soepcr方法在感染性分子克隆载体的s2基因独特区内引入突变点，形成含有酶切位点（ nspv ）的突变体（ p1p4 ） 。 |
| 10. | The donkey - adapted strain of eiav ( dv116 ) , parental strain of eiav - dla , is a highly virulent isolate which was developed by sequential passaging a virulent eiav strain in donkeys in 1970s . the donkeys inoculated with fatal doses of eiav d strain can always be killed within in the first acute disease period . in this study , we constructed a full - length provirus dna clone of dv116 by pcr
本实验中将eiav驴强毒dv体外感染驴白细胞培养物，一定时间后收获病毒(本文中简称dv116 ) ，提取eiav前病毒dna ，以pcr法分别扩增并克隆了包含全长基因的三段前病毒dna片段，以双脱氧法测定了dv116病毒全基因序列共8236个核苷酸。 |
