| 1. | However all of these data come from in vitro studies
尚未见对人elongator复合物及其亚基功能的体内研究的报道。 |
| 2. | The rnapii - associated complex elongator has histone acetyltransferase ( hat ) activity and thus might fulfil such a role
与磷酸化的rnap结合的elongator复合物具有组蛋白乙酰转移酶( hat )活性，它可能执行这一功能。 |
| 3. | Mutations that debilitate the hat activity of eip3 in vitro also confer elp phenotypes in vivo , indicating that the hat activity of elongator is required for its function
Elp3具有hat活性，该活性为复合物行使功能所必需。使elp3的hat活性严重丧失的点突变可引起所有elp表型。 |
| 4. | The activity of intact elongator complex is directed specifically toward the amino - terminal tails of histone h3 and h4 , the predominant acetylation sites are lysine - 14 of histone h3 and lysine - 8 of histone h4
完整的复合物可直接修饰组蛋白h3和h4尾部，其优先乙酰化位点是h3k14和h4k8 。 |
| 5. | There have been no reports so far on the characterization of human elongator complex in vivo . in this thesis , we studied the function of hat activity of human elongator complex in gene expression and regulation in saccharomyces cerevisiae
证明了人与酵母的elp3在功能上是保守的，初步确定了help3及其拥有的hat活性在复合物中的功能地位，了解了复合物hat活性在基因表达调控中的作用方式。 |
| 6. | Yeast elongator was isolated as a complex that associates with the chromatin fraction and interacts with the elongating phosphorylated form of rnap ii . the functional entity of elongator complex has recently been shown to be an unstable six - subunit complex , termed holo - elongator , which can dissociate into two discrete three - subunit subcomplexes upon treatment with high salt and / or monoq chromatography . one of these subcompexes is the elp3 - containing core complex , and the other is a complex of the elp4 , elp5 and elp6 proteins
酵母elongator是与染色质组分结合，并与延伸中的磷酸化形式的rnap相互作用的复合物，是个六亚基的功能整体，在高盐处理或monoq层析时易于分解为两个三亚基亚复合物，其中一个是含elp3的核心复合物，另一个含elp4 5 6亚基。 |
| 7. | Human elongator complex serves a role in chromatin remodeling through modifying the lysine residues of histone h3 and h4 during transcription elongation in higher cells . the main results and conclusions are as followings . 1 using depletion strain of yeast as material , we have established a system for complementation test and gene expression analysis of human elongator complex
4 、利用点突变和plasmidshuffle等技术构建了含h3k14一r 、 h4ks ~ r单突变和h3k14一侧h4ks一r双突变的e1p3乙菌株，通过功能互补实验分析少凡p3对突变株的补偿功能，证实酵母elongator可对h314k / h4sk进行乙酞化修饰。 |
| 8. | Recently , human elongator complexes have been isolated . the human complex is a fragile six - subunit complex that interacts with rnap ii and has hat activity directed against histone h3 and h4 . depletion of elongator from hela nuclear extracts reduces the ability of these extracts to transcribe chromatin templates , providing biochemical support for the proposed role of elongator during transcript elongation in chromatin
近来人elongator也被分离，它也是个不稳定的六亚基复合物，可与rnap相互作用，具有hat活性，能乙酰化h3和h4 ，在hela细胞核抽提物中去除elongator可降低抽提物对染色质模板的转录延伸速率，提供了该复合物在染色质为模板的转录延伸中有功能的生化证据，但是上面的信息都来自体外研究。 |
